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dc.contributorWichita State University. Department of Chemistryen_US
dc.contributor.authorSinghal, Ram P.en_US
dc.contributor.authorHughbanks, D.en_US
dc.contributor.authorXian, Junen_US
dc.identifier.citationJournal of chromatography. 1992 Sep 18; 609(1-2): 147-61.en_US
dc.descriptionFull text of this article is not available in SOAR.en_US
dc.description.abstractNo satisfactory high-performance liquid chromatographic (HPLC) method is currently available for the separation of the major dideoxyribonucleosides (ddNs) and their derivatives. A method involving HPLC has been developed for the separation of five major ddNs [ddA, ddC, ddI, azT and 2',3'-dideoxy-2',3'-didehydrothymidine (d4T)]. Elution of the common and modified components of DNA was also examined under the selected separation conditions of HPLC. The elution characteristics of these compounds were studied using serum plasma samples spiked with ddN derivatives. In addition, capillary electrophoresis (CE) was investigated for the separation of ddNs and their derivatives. Picomolar amounts of the five major ddNs and the metabolic product of azT [5'-O-glucuronide-3'-azido-3'-deoxythymidine (Glo-azT)] were satisfactorily resolved in 10 min by using a modification of CE. The spectral properties of the ddNs were characterized under different pH conditions and compared with those of their parent deoxyribonucleosides (dNs) because these compounds are commonly measured in HPLC by their spectral properties. The spectra of ddC and ddT derivatives resemble very closely those of dC and dT, but those of ddA and ddI differ to some extent from their parent dNs. The HPLC method was extensively examined for satisfactory resolutions of these compounds. For example, an isocratic elution method, although simple, failed to resolve these compounds and ion-pair chromatography did not offer any advantage. Gradient elution involving buffered solutions and increasing amounts of an organic modifier yielded satisfactory results. Methanol appeared to be the organic modifier of choice. A reversed-phase matrix with smaller than octadecyl alkyl chains did not produce the necessary interactions. Uniform spherical beads of smaller diameter produced superior resolutions. The separation of these compounds on three commercially available columns is discussed. The separation of human plasma samples spiked with dideoxynucleoside derivatives by HPLC was accomplished in ca. 16 min. The presence of the dNs did not interfere in their separations.en_US
dc.relation.ispartofseriesJournal of chromatographyen_US
dc.relation.ispartofseriesJ. Chromatogr.en_US
dc.subject.meshCapillary Actionen_US
dc.subject.meshChromatography, High Pressure Liquid/methodsen_US
dc.subject.meshDNA/isolation & purificationen_US
dc.subject.meshDideoxynucleosides/isolation & purificationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.titleSeparation of dideoxyribonucleosides in trace amounts by automated liquid chromatography and capillary electrophoresisen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 1992, Elsevieren_US

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