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dc.contributor.authorSchneegurt, Mark A.en_US
dc.date.accessioned2012-01-24T17:49:38Z
dc.date.available2012-01-24T17:49:38Z
dc.date.issued2005en_US
dc.identifier15812861en_US
dc.identifier8503885en_US
dc.identifier.citationJournal of basic microbiology. 2005; 45(2): 155-9.en_US
dc.identifier.issn0233-111Xen_US
dc.identifier.urihttp://dx.doi.org/10.1002/jobm.200410479
dc.identifier.urihttp://hdl.handle.net/10057/4191
dc.descriptionClick on the DOI link below to access the article (may not be free).en_US
dc.description.abstractA biosynthetic precursor of tetrapyrroles, delta-aminolevulinic acid (ALA), can be formed via two pathways: enzymatic condensation of glycine and succinyl-CoA by ALA synthase in animal mitochondria and some fungi, and the C5 pathway converting glutamate to ALA in plants, algae, archaea, and most bacteria. The two pathways are distinguishable using specifically radiolabeled compounds. The C1 of glutamate is lost during conversion to succinate in the TCA cycle, and the C2 of glycine is lost during conversion to acetyl-CoA on the way to glutamate. Desalted high-speed supernatants of Ustilago maydis sporidia extracts were assayed using specifically radiolabeled substrates. A significant amount of radiolabel was incorporated into ALA from 2-[14C]glycine. No radiolabel was incorporated into ALA from 1-[14C]glutamate. These results indicate that the basidiomycete yeast, Ustilago maydis, has active ALA synthase.en_US
dc.language.isoengen_US
dc.publisherWiley - V C H Verlag GmbH & Co. KGaAen_US
dc.relation.ispartofseriesJournal of basic microbiologyen_US
dc.sourceNLMen_US
dc.subject.mesh5-Aminolevulinate Synthetase/metabolismen_US
dc.subject.meshAminolevulinic Acid/metabolismen_US
dc.subject.meshCarbon Radioisotopesen_US
dc.subject.meshGlycine/metabolismen_US
dc.subject.meshSubstrate Specificityen_US
dc.subject.meshUstilago/metabolismen_US
dc.titleDelta-aminolevulinic acid biosynthesis in Ustilago maydisen_US
dc.typeArticleen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_US


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