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dc.contributor.authorNguyen, Van T.en_US
dc.contributor.authorSingh, Vinoden_US
dc.contributor.authorButnev, Vladimir Y.en_US
dc.contributor.authorGray, Ciann M.en_US
dc.contributor.authorWestfall, Suzanneen_US
dc.contributor.authorDavis, John S.en_US
dc.contributor.authorDias, James A.en_US
dc.contributor.authorBousfield, George R.en_US
dc.date.accessioned2012-01-24T17:49:35Z
dc.date.available2012-01-24T17:49:35Z
dc.date.issued2003-01-31en_US
dc.identifier12581881en_US
dc.identifierAG15428/ DK52383en_US
dc.identifier7500844en_US
dc.identifierS0303720702002976en_US
dc.identifier.citationMolecular and cellular endocrinology. 2003 Jan 31; 199(1-2): 73-86.en_US
dc.identifier.issn0303-7207en_US
dc.identifier.urihttp://dx.doi.org/10.1016/S0303-7207(02)00297-6
dc.identifier.urihttp://hdl.handle.net/10057/4183
dc.descriptionClick on the DOI link below to access the article (may not be free).en_US
dc.description.abstractLentil lectin-bound, fucose-enriched hTSH was reported to stimulate both cAMP and inositol phosphate (IP) intracellular signalling pathways, whereas fucose-depleted hTSH stimulated only the cAMP pathway. Gonadotropins activate the cAMP pathway and in several studies higher concentrations activate the IP pathway. Since only the 10% of alpha subunit Asn(56) oligosaccharides (Asn(52) in humans) are fucosylated, the higher glycoprotein hormone concentrations required for IP pathway activation might be related to the abundance of competent hormone isoforms. Lentil lectin-fractionated equine (e)LHalpha and eFSHalpha preparations were combined with a truncated, des(121-149)eLHbeta preparation. All four hybrid hormone preparations induced IP accumulation in porcine theca cells, suggesting that activation of the IP pathway was not dependent on fucosylation at alpha subunit Asn(56). However, the presence of Asn(56) carbohydrate was necessary for increased IP accumulation. Intact, rather than Asn(56)-deglycosylated eLH preparations provoked a biphasic steroidogenic response by rat testis Leydig cells, suggesting that Galpha(i) stimulation was also sensitive to loss of Asn(56) carbohydrate. While rat granulosa cells responded to human FSH preparations in a biphasic manner, a classical sigmoidal response was obtained to eFSH and Asn(56)-deglycosylated eFSH, suggesting that the equine preparations did not activate Galpha(i). Purified oLHalpha Asn(56) oligosaccharides inhibited FSH-stimulated steroidogenesis in rat granulosa cell cultures indicating a direct role for carbohydrate in FSH action. The same carbohydrate preparation inhibited hCG-stimulated fluorescence energy transfer suggesting oligosaccharide involvement in activated LH receptor self-association.en_US
dc.description.sponsorshipNIA NIH HHS/ NIDDK NIH HHSen_US
dc.language.isoengen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofseriesMolecular and cellular endocrinologyen_US
dc.sourceNLMen_US
dc.subjectResearch Support, Non-U.S. Gov'ten_US
dc.subjectResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subjectResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshAsparagineen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFemaleen_US
dc.subject.meshFollicle Stimulating Hormone/pharmacologyen_US
dc.subject.meshFucose/metabolismen_US
dc.subject.meshGlycosylationen_US
dc.subject.meshGranulosa Cells/metabolismen_US
dc.subject.meshHorsesen_US
dc.subject.meshHumansen_US
dc.subject.meshInositol Phosphates/metabolismen_US
dc.subject.meshLeydig Cells/metabolismen_US
dc.subject.meshLuteinizing Hormone/chemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshOligosaccharides/chemistryen_US
dc.subject.meshRatsen_US
dc.subject.meshSignal Transductionen_US
dc.subject.meshSteroids/biosynthesisen_US
dc.subject.meshStructure-Activity Relationshipen_US
dc.subject.meshSwineen_US
dc.subject.meshTheca Cells/metabolismen_US
dc.subject.meshLuteinizing Hormone/pharmacologyen_US
dc.subject.meshOligosaccharides/pharmacologyen_US
dc.titleInositol phosphate stimulation by LH requires the entire alpha Asn56 oligosaccharideen_US
dc.typeArticleen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 2003, Elsevieren_US


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