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dc.contributor.authorSweet, George H.en_US
dc.contributor.authorDulohery, Susan M.en_US
dc.identifier.citationThe American review of respiratory disease. 1989 Oct; 140(4): 967-73.en_US
dc.descriptionClick on the link below to access the article (may not be free).en_US
dc.description.abstractBiotin-avidin enzyme-linked immunosorbent assays (ELISA) were developed to study the appearance of free antibody of the IgM, G, and A classes of immune complexes (IC) containing those classes and of antigen in the serum and urine of mice with experimentally induced, disseminated histoplasmosis (histo) over a period ranging from 4 to 64 days after infection. Free IgM was detected 4 days after infection, remained at low levels, and disappeared on Day 64, whereas free IgG was first detected on Day 7 and rose to very high levels before declining on Day 64. Free IgA was detected only once, on Day 21. IC containing IgM were seen first on Day 4, remained at low levels, and became undetectable on Day 64. IC containing IgM were detected on Day 7, peaked on Day 14, and declined through Day 64. IC containing IgA wee seen at low levels on Days 7, 14, and 21. Estimation of total IgA levels, done by single radial immunodiffusion, showed a statistically significant decrease on Day 14, followed by a slightly significant increase on Days 21 and 30. Antigen was detected in as much as 80% of serum specimens and 100% of urine samples during the first 2 wk postinfection but rarely afterwards. We conclude that ELISA provides a highly sensitive way to study antibody, IC, and antigen in host body fluids during histo infection and that IgM and antigen detection can be very early indices of infection. Measurements of IC and total IgA seem to be of relatively less importance in detection of infection.en_US
dc.publisherAmerican Thoracic Societyen_US
dc.relation.ispartofseriesThe American review of respiratory diseaseen_US
dc.subject.meshAntibodies, Fungal/analysisen_US
dc.subject.meshAntigen-Antibody Complex/analysisen_US
dc.subject.meshAntigens, Fungal/analysisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshImmunoglobulin A/analysisen_US
dc.subject.meshImmunoglobulin G/analysisen_US
dc.subject.meshImmunoglobulin M/analysisen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshAntibodies, Fungal/classificationen_US
dc.subject.meshAntigens, Fungal/urineen_US
dc.titleExperimental histoplasmosis in the mouse. Immunoglobulin class response, total immunoglobulin A levels, immune complex formation, and occurrence of antigen in serum and urineen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright @ American Thoracic Societyen_US

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