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dc.contributor.authorAinsworth, Samuel M.en_US
dc.contributor.authorNagy, Stephanie B.en_US
dc.contributor.authorMorgan, Loretta A.en_US
dc.contributor.authorMiller, Glendon R.en_US
dc.contributor.authorPerry, Jack L.en_US
dc.date.accessioned2012-01-24T17:48:58Z
dc.date.available2012-01-24T17:48:58Z
dc.date.issued1990-11en_US
dc.identifier1701446en_US
dc.identifier7505564en_US
dc.identifier.citationJournal of clinical microbiology. 1990 Nov; 28(11): 2559-60.en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://ukpmc.ac.uk/articles/PMC268224/pdf/jcm00059-0191.pdf
dc.identifier.urihttp://hdl.handle.net/10057/4145
dc.descriptionClick on the link below to access the article (may not be free).en_US
dc.description.abstractSputum specimens culture positive for Moraxella (Branhamella) catarrhalis were Gram stained with three decolorizer solutions (slow, 95% ethyl alcohol; intermediate, 1:1 ratio of 95% ethyl alcohol and acetone; and fast, acetone alone) for 5, 10, 20, and 30 s. Optimal results were obtained with acetone alone after 10 s or with a 1:1 mixture of acetone and ethanol after 20 s. Inadequate decolorization of M. catarrhalis in sputa is likely if the decolorization solution and exposure time are not optimal and may contribute to underreporting of this organism.en_US
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofseriesJournal of clinical microbiologyen_US
dc.sourceNLMen_US
dc.subject.meshEvaluation Studies as Topicen_US
dc.subject.meshGentian Violeten_US
dc.subject.meshHumansen_US
dc.subject.meshMoraxella (Branhamella) catarrhalis/isolation & purificationen_US
dc.subject.meshPhenazinesen_US
dc.subject.meshSolutionsen_US
dc.subject.meshSputum/microbiologyen_US
dc.subject.meshStaining and Labeling/methodsen_US
dc.titleInterpretation of gram-stained sputa containing Moraxella (Branhamella) catarrhalisen_US
dc.typeArticleen_US
dc.description.versionpeer revieweden_US
dc.rights.holderCopyright © 1990, American Society for Microbiologyen_US


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