Browsing CHEM Faculty Scholarship by Author "Bann, James G."
Now showing items 1-20 of 25
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19F nuclear magnetic resonance and crystallographic studies of 5-fluorotryptophan-labeled anthrax protective antigen and effects of the receptor on stability
Chadegani, Fatemeh; Lovell, Scott; Mullangi, Vennela; Miyagi, Masaru; Battaile, Kevin P.; Bann, James G. (American Chemical Society, 2014-02-04)The anthrax protective antigen (PA) is an 83 kDa protein that is one of three protein components of the anthrax toxin, an AB toxin secreted by Bacillus anthracis. PA is capable of undergoing several structural changes, ... -
2-Fluoro-l-histidine
Andra, Kiran K.; Bullinger, John C.; Bann, James G.; Eichhorn, David M. (International Union of Crystallography, 2010-01-01)The title compound, C(6)H(8)FN(3)O(2), an analog of histidine, shows a reduced side-chain pK(a) (ca 1). The title structure exhibits a shortening of the bond between the proximal ring N atom and the F-substituted ring C ... -
3S-fluoroproline as a probe to monitor proline isomerization during protein folding by 19F-NMR
Thomas, Colin A.; Talaty, Erach R.; Bann, James G. (Royal Society of Chemistry, 2009-06-21)Variable-temperature inversion transfer NMR is used to determine the kinetic and thermodynamic parameters of cis-trans isomerization of N-Ac-(3R) and (3S)-fluoroproline-OMe. -
Advances in structure determination by cryo-EM to unravel membrane-spanning pore formation
Scott, Harry; Huang, Wei; Bann, James G.; Taylor, Derek J. (John Wiley and Sons, 2018-08-20)The beta pore-forming proteins (-PFPs) are a large class of polypeptides that are produced by all Kingdoms of life to contribute to their species' own survival. Pore assembly is a sophisticated multi-step process that ... -
Anthrax toxin protective antigenuInsights into molecular switching from prepore to pore
Bann, James G. (Cambridge University Press, 2012-01)The protective antigen is a key component of the anthrax toxin, as it allows entry of the enzymatic components edema factor and lethal factor into the host cell, through the formation of a membrane spanning pore. This event ... -
Binding of the von Willebrand factor A domain of capillary morphogenesis protein 2 to anthrax protective antigen vaccine reduces immunogenicity in mice
de Oliveira, Fabiana Freire Mendes; Mamillapalli, Sireesha; Gonti, Srinivas; Brey, Robert N.; Li, Han; Schiffer, Jarad M.; Casadevall, Arturo; Bann, James G. (NLM (Medline), 2020-01)Protective antigen (PA) is a component of anthrax toxin that can elicit toxin-neutralizing antibody responses. PA is also the major antigen in the current vaccine to prevent anthrax, but stability problems with recombinant ... -
Biophysical characterization and immunization studies of dominant negative inhibitor (DNI), a candidate anthrax toxin subunit vaccine
Iyer, Vidyashankara; Hu, Lei; Schante, Carole E.; Vance, David; Chadwick, Chrystal; Jain, Nishant Kumar; Brey, Robert N.; Joshi, Sangeeta B.; Volkin, David B.; Andra, Kiran K.; Bann, James G.; Mantis, Nicholas J.; Middaugh, C. Russell (Landes Bioscience, 2013-11)Dominant negative inhibitor (DNI) is a translocation-deficient homolog of recombinant protective antigen of Bacillus anthracis that is a candidate for a next generation anthrax vaccine. This study demonstrates that the ... -
The biophysical probes 2-fluorohistidine and 4-fluorohistidine: spectroscopic signatures and molecular properties
Kasireddy, Chandana; Ellis, Jonathan M.; Bann, James G.; Mitchell-Koch, Katie R. (Nature Publishing Group, 2017-02-15)Fluorinated amino acids serve as valuable biological probes, by reporting on local protein structure and dynamics through F-19 NMR chemical shifts. 2-fluorohistidine and 4-fluorohistidine, studied here with DFT methods, ... -
Biosynthetic incorporation of fluorohistidine into proteins in E. coli: a new probe of macromolecular structure
Eichler, Jack F.; Cramer, John C.; Kirk, Kenneth L.; Bann, James G. (John Wiley and Sons, 2005-12-01) -
Catalysis of protein folding by chaperones in pathogenic bacteria
Bann, James G.; Pinkner, Jerome S.; Frieden, Carl; Hultgren, Scott J. (2004-12-14)Molecular chaperones are thought to inhibit off-pathway interactions such as aggregation from occurring without influencing the on-pathway formation of native structure. Here, we present a mechanism whereby the family of ... -
Comparison of the structural stability and dynamic properties of recombinant anthrax protective antigen and its 2-fluorohistidine-labeled analogue
Hu, Lei; Joshi, Sangeeta B.; Andra, Kiran K.; Thakkar, Santosh V.; Volkin, David B.; Bann, James G.; Middaugh, C.R. (Wiley-Blackwell, 2012-11)Protective antigen (PA) is the primary protein antigenic component of both the currently used anthrax vaccine and related recombinant vaccines under development. An analogue of recombinant PA (2-FHis rPA) has been recently ... -
Demystifying fluorine chemical shifts: electronic structure calculations address origins of seemingly anomalous F-19-NMR spectra of fluorohistidine isomers and analogues
Kasireddy, Chandana; Bann, James G.; Mitchell-Koch, Katie R. (Royal Society of Chemistry, 2015-10-23)Fluorine NMR spectroscopy is a powerful tool for studying biomolecular structure, dynamics, and ligand binding, yet the origins of F-19 chemical shifts are not well understood. Herein, we use electronic structure calculations ... -
Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH
Williams, Alexander S.; Lovell, Scott; Anbanandam, Asokan; El-Chami, Rahif; Bann, James G. (John Wiley and Sons, 2009-11)Domain 4 of the anthrax protective antigen (PA) plays a key role in cellular receptor recognition as well as in pH-dependent pore formation. We present here the 1.95 A crystal structure of domain 4, which adopts a fold ... -
Effect of 2-fluorohistidine labeling of the anthrax protective antigen on stability, pore formation, and translocation
Wimalasena, D. Shyamali; Cramer, John C.; Janowiak, Blythe E.; Juris, Stephen J.; Melnyk, Roman A.; Anderson, David E.; Kirk, Kenneth L.; Collier, R. John; Bann, James G. (American Chemical Society, 2007-12-25)The action of anthrax toxin relies in part upon the ability of the protective antigen (PA) moiety to form a heptameric pore in the endosomal membrane, providing a portal for entry of the enzymic moieties of the toxin into ... -
Electronic structure calculations of fluorohistidine isomers
Kasireddy, Chandana; Bann, James G.; Mitchell-Koch, Katie R. (American Chemical Society, 2014-08-10)This work assesses the stability and NMR shifts of fluorinated amino acids in different solvents. We have considered the τ and π tautomers for both 2-fluorohistidine (2F-his) and 4-fluorohistidine (4F-his) corresponding ... -
Evidence that histidine protonation of receptor-bound anthrax protective antigen is a trigger for pore formation
Wimalasena, D. Shyamali; Janowiak, Blythe E.; Lovell, Scott; Miyagi, Masaru; Sun, Jianjun; Zhou, Haiying; Hajduch, Jan; Pooput, Chaya; Kirk, Kenneth L.; Battaile, Kevin P.; Bann, James G. (American Chemical Society, 2010-08-24)The protective antigen (PA) component of the anthrax toxin forms pores within the low pH environment of host endosomes through mechanisms that are poorly understood. It has been proposed that pore formation is dependent ... -
Long-range stabilization of anthrax protective antigen upon binding to CMG2
Mullangi, Vennela; Mamillapalli, Sireesha; Anderson, David J.; Bann, James G.; Miyagi, Masaru (American Chemical Society, 2014-09-30)Protective antigen (PA) mediates entry of edema factor (EF) and lethal factor (LF) into the cytoplasmic space of the cells through the formation of a membrane-spanning pore. To do this, PA must initially bind to a host ... -
LRP6 holds the key to the entry of anthrax toxin
Bann, James G.; Cegelski, Lynette; Hultgren, Scott J. (Elsevier, 2006-03-24)In this issue of Cell, it is demonstrated that the low-density lipoprotein receptor-related protein 6 (LRP6) promotes endocytosis of the anthrax toxin into cells. LRP6 acts as a coreceptor with either TEM8 or CMG2, the two ... -
Monitoring anthrax toxin receptor dissociation from the protective antigen by NMR
Rajapaksha, Maheshinie; Eichler, Jack F.; Hajduch, Jan; Anderson, David E.; Kirk, Kenneth L.; Bann, James G. (John Wiley and Sons, 2009-01-01)The binding of the Bacillus anthracis protective antigen (PA) to the host cell receptor is the first step toward the formation of the anthrax toxin, a tripartite set of proteins that include the enzymatic moieties edema ... -
Periplasmic peptidyl prolyl cis-trans isomerases are not essential for viability, but SurA is required for pilus biogenesis in Escherichia coli
Justice, Sheryl S.; Hunstad, David A.; Harper, Jill R.; Duguay, Amy R.; Pinkner, Jerome S.; Bann, James G.; Frieden, Carl; Silhavy, Thomas J.; Hultgren, Scott J. (American Society for Microbiology, 2005-08-31)In Escherichia coli, FkpA, PpiA, PpiD, and SurA are the four known periplasmic cis-trans prolyl isomerases. These isomerases facilitate proper protein folding by increasing the rate of transition of proline residues between ...