Browsing CHEM Faculty Scholarship by Title
Now showing items 361-380 of 496
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Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy
(Springer New York, 2015)Nuclear magnetic resonance (NMR) has continued to evolve as a powerful method, with an increase in the number of pulse sequences and techniques available to study protein-protein interactions. In this chapter, a straightforward ... -
Prototyping of poly(dimethylsiloxane) interfaces for flow gating, reagent mixing, and tubing connection in capillary electrophoresis
(Elsevier B.V., 2014-01-10)Integrated microfluidic systems coupled with electrophoretic separations have broad application in biologic and chemical analysis. Interfaces for the connection of various functional parts play a major role in the performance ... -
Purification and analytical assessment of the mycobacterial porin MspA and investigation of its channel activity and potential use for a protein nano-solar cell
(American Chemical Society, 2011-08-28) -
Putative structural rearrangements associated with the interaction of macrocyclic inhibitors with norovirus 3CL protease
(John Wiley and Sons, 2019-03-18)Human noroviruses are the primary cause of outbreaks of acute gastroenteritis worldwide. The problem is further compounded by the current lack of norovirus-specific antivirals or vaccines. Noroviruses have a single-stranded, ... -
Quantitative assessment of additive leachates in abiotic weathered tire cryogrinds and its application to tire wear particles in roadside soil samples
(Elsevier Ltd, 2023-01-01)Tire and road wear particles (TRWP) are becoming an important research question with potential risks on ecological system. A comprehensive understanding of their detection and quantification in soils are challenged by the ... -
Quasi-layered crystal structure coupled with point defects leading to ultralow lattice thermal conductivity in n-type $CU_{2.83}Bi_{10}Se_{16}$
(American Chemical Society, 2021-10-01)$CU_{2.83}Bi_{10}Se_{16}$ a new n-type thermoelectric material, was synthesized via a high-temperature solid-state routine. The quasi-layered structure features of $CU_{2.83}Bi_{10}Se_{16}$ were established by a comprehensive ... -
Rapid amino acid analysis of beers using flow-gated capillary electrophoresis coupled with side-by-side calibration
(Royal Society of Chemistry, 2017-08-21)Amino acid analysis is an important procedure to measure free amino acids (AAs) in various samples including proteins, blood plasma, urine, foods and beverages. Due to the complexity of these samples, chemical separations ... -
Rapid determination of free prolyl dipeptides and 4-hydroxyproline in urine using flow-gated capillary electrophoresis
(Springer, 2017-12)Unhydrolyzed prolyl hydroxyproline (Pro-Hyp) and total 4-hydroxyproline (Hyp) in urine have been suggested as disease biomarkers for bone turnover and osteoporosis. Here, a rapid method was developed to accurately and ... -
Rapid labeling of amino acid neurotransmitters with a fluorescent thiol in the presence of o-phthalaldehyde
(John Wiley & Sons, Inc., 2017-02)LIF detection often requires labeling of analytes with fluorophores; and fast fluorescent derivatization is valuable for high-throughput analysis with flow-gated CE. Here, we report a fast fluorescein-labeling scheme for ... -
Rational approach to selective and direct 2-O-alkylation of 5,6-O-isopropylidine-L-ascorbic acid
(American Chemical Society, 2004-10-15)l-Ascorbic acid is a versatile radical scavenger widely distributed in aerobic organisms that plays a central role in the protection of cellular components against oxidative damage by free radicals and oxidants. It also ... -
The rational synthesis of chlorins via rearrangement of porphodimethenes: influence of beta-substituents on the regioselectivity and stereoselectivity of pyrroline ring formation
(American Chemical Society, 2002-06-28)The porphodimethene rearrangement methodology reported in this paper provides for a rational, step-by-step synthesis of chlorins from readily available pyrrole precursors. The intermediate porphodimethenes are furnished ... -
Recent advances in the discovery of norovirus therapeutics
(American Chemical Society, 2015-12-24)Noroviruses are members of the family Caliciviridae. Norovirus infections are a global health burden that impacts >20 million individuals annually in the U.S. alone. Noroviruses are associated with high morbidity among ... -
Recent developments in the design of mechanism-based and alternate substrate inhibitors of serine proteases
(Bentham Science, 2004-01-01)A wide range of human diseases are associated with the aberrant activity of mammalian, viral, bacterial or parasitic proteases. These include members of all four classes of proteases, namely, serine, cysteine, aspartic and ... -
Recommendations for reporting ion mobility mass spectrometry measurements
(Wiley, 2019-03-07)Here we present a guide to ion mobility mass spectrometry experiments, which covers both linear and nonlinear methods: what is measured, how the measurements are done, and how to report the results, including the uncertainties ... -
Reduction of dopamine beta-monooxygenase. A unified model for apparent negative cooperativity and fumarate activation
(American Society for Biochemistry and Molecular Biology, 1996-10-18)The interactions of reductants with dopamine beta-monooxygenase (DbetaM) were examined using two novel classes of reductants. The steady-state kinetics of the previously characterized DbetaM reductant, N,N-dimethyl-1,4-p ... -
The reduction of membrane-bound dopamine beta-monooxygenase in resealed chromaffin granule ghosts. Is intragranular ascorbic acid a mediator for extragranular reducing equivalents?
(American Society for Biochemistry and Molecular Biology, 1995-11-17)The role of internal and external reductants in the dopamine beta-monooxygenase (D beta M)-catalyzed conversion of dopamine to norepinephrine has been investigated in resealed chromaffin granule ghosts. The rate of ... -
Repurposing an inhibitor of ribosomal biogenesis with broad anti-fungal activity
(Nature Publishing Group, 2017-12-05)The lack of new antifungal compounds with unique mechanisms of action is a concern for therapeutic management of patients. To identify inhibitors against human pathogenic fungi, we screened similar to 3000 compounds provided ... -
Results compared for tricyclic antidepressants as assayed by liquid chromatography and enzyme immunoassay
(P.B. Hoeber, 1988-11-01)The tricyclic antidepressants amitriptyline, nortriptyline, imipramine, and desipramine in serum of patients taking one of the drugs were quantified in two laboratories by high-performance liquid chromatography (HPLC) and ... -
Reversal of the progression of fatal coronavirus infection in cats by a broad-spectrum coronavirus protease inhibitor
(Public Library of Science Pathogens, 2016-03-30)Coronaviruses infect animals and humans causing a wide range of diseases. The diversity of coronaviruses in many mammalian species is contributed by relatively high mutation and recombination rates during replication. This ... -
Review -- Two different multiple photosynthetic reaction centers using either zinc porphyrinic oligopeptide-fulleropyrrolidine or free-base porphyrinic polypeptide-Li+@C60supramolecular complexes
(Institute of Physics Publishing, 2020-08-10)An overview of two successful examples of photosynthetic reaction center models combined with light-capturing antenna chromophores is presented. In the first example, supramolecular complexes are formed between flexible ...