• Login
    View Item 
    •   Shocker Open Access Repository Home
    • Engineering
    • Mechanical Engineering
    • ME Research Publications
    • View Item
    •   Shocker Open Access Repository Home
    • Engineering
    • Mechanical Engineering
    • ME Research Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Synthesis and evaluation of electrospun PCL-plasmid DNA nanofibers for post cancer treatments

    Date
    2022-08-12
    Author
    Ceylan, Muhammet
    Asmatulu, Ramazan
    Jiang, Jianhao
    Usta, Aybala
    Jia, Tanghong
    Yao, Li
    Yang, Shang-you
    Metadata
    Show full item record
    Citation
    Ceylan, M., Asmatulu, R., Jiang, J., Usta, A., Jia, T., Yao, L., & Yang, S.-y. (2022). Synthesis and evaluation of electrospun PCL-plasmid DNA nanofibers for post cancer treatments. Materials Today: Proceedings. https://doi.org/https://doi.org/10.1016/j.matpr.2022.07.447
    Abstract
    Poly-?-caprolactone (PCL) incorporated with plasmid DNA was electrospun, and the resultant nanofibers were used to observe DNA release from the nanofibers. The plasmid DNA enhanced green fluorescent protein (EGFP) with cytomegalovirus (CMV) promoter (PCMVb-GFP) was amplified with E. coli. PCL was chosen because it is biodegradable aliphatic polyester, which plays a critical role in tissue engineering, such as scaffolding, drug, DNA, gene and protein delivery vehicles. Some of the physical and biological properties of the nanofibers were determined using different methods. Scanning electron microscopy (SEM) micrographs showed that nanofibers have an average diameter of about 100 nm. Cytotoxicity tests showed that cell viability for 1 day, 4 days, and 7 days of the tests were above 80 %. These data demonstrated that PCL-plasmid DNA nanofibers have no cytotoxicity and showed benign biocompatibility for biomedical applications. PCMVb-GFP plasmid-linked electrospun nanofibers continuously released double-stranded DNA for at least seven days. For the first 15 min, there was a burst release of about 1.8 ng/ml. For the following hours and days, the release was about to be the same (release of 0.575 ng/ml). Therefore, PCL nanofibers may be an ideal candidate for various biomedical applications such as cancer treatment, scaffolding, tissue engineering, and protein delivery vehicles. © 2022
    Description
    Click on the DOI to access this article (may not be free).
    URI
    https://doi.org/10.1016/j.matpr.2022.07.447
    https://soar.wichita.edu/handle/10057/23933
    Collections
    • ME Research Publications

    Browse

    All of Shocker Open Access RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsBy TypeThis CollectionBy Issue DateAuthorsTitlesSubjectsBy Type

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV