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dc.contributor.authorPolk, Tabatha
dc.contributor.authorSchmitt, Sarah
dc.contributor.authorAldrich, Jessica
dc.contributor.authorLong, David S.
dc.date.accessioned2022-06-13T15:04:35Z
dc.date.available2022-06-13T15:04:35Z
dc.date.issued2022-06-02
dc.identifier.citationPolk, T., Schmitt, S., Aldrich, J. L., & Long, D. S. (2022). Human dermal microvascular endothelial cell morphological response to fluid shear stress. Microvascular Research, 143, 104377. https://doi.org/https://doi.org/10.1016/j.mvr.2022.104377
dc.identifier.issn0026-2862
dc.identifier.urihttps://doi.org/10.1016/j.mvr.2022.104377
dc.identifier.urihttps://soar.wichita.edu/handle/10057/23427
dc.descriptionClick on the DOI to access this article (may not be free).
dc.description.abstractAs the cells that line the vasculature, endothelial cells are continually exposed to fluid shear stress by blood flow. Recent studies suggest that the morphological response of endothelial cells to fluid shear stress depends on the endothelial cell type. Thus, the present study characterizes the morphological response of human dermal microvascular endothelial cells (HMEC-1) and nuclei to steady, laminar, and unidirectional fluid shear stress. Cultured HMEC-1 monolayers were exposed to shear stress of 0.3 dyn/cm2, 16 dyn/cm2, or 32 dyn/cm2 for 72 h with hourly live-cell imaging capturing both the nuclear and cellular morphology. Despite changes in elongation and alignment occurring with increasing fluid shear stress, there was a lack of elongation and alignment over time under each fluid shear stress condition. Conversely, changes in cellular and nuclear area exhibited dependence on both time and fluid shear stress magnitude. The trends in cellular morphology differed at shear stress levels above and below 16 dyn/cm2, whereas the nuclear orientation was independent of fluid shear stress magnitude. These findings show the complex morphological response of HMEC-1 to fluid shear stress.
dc.description.sponsorshipThis work was supported by startup funds from the College of Engineering at Wichita State University (D.S.L.). T.P., S.S., and J.L.A. received partial support from the Kansas Idea Network of Biomedical Research Excellence , (Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103418 ). T.P. received summer research support from the College of Engineering at Wichita State University .
dc.language.isoen_US
dc.publisherElsevier
dc.relation.ispartofseriesMicrovascular Research
dc.relation.ispartofseriesVol. 143
dc.subjectHuman dermal microvascular endothelial cells
dc.subjectMechanobiology
dc.subjectCell morphology
dc.subjectNuclear morphology
dc.subjectFluid shear stress
dc.subjectLive-cell imaging
dc.titleHuman dermal microvascular endothelial cell morphological response to fluid shear stress
dc.typeArticle
dc.rights.holder© 2022 Elsevier Inc. All rights reserved.


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