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dc.contributor.authorSun, Renyi
dc.contributor.authorJia, Tanghong
dc.contributor.authorDart, Bradley
dc.contributor.authorShrestha, Sunaina
dc.contributor.authorBretches, Morgan
dc.contributor.authorHeggeness, Michael H.
dc.contributor.authorYang, Shang-You
dc.date.accessioned2021-10-22T18:45:49Z
dc.date.available2021-10-22T18:45:49Z
dc.date.issued2021-09-26
dc.identifier.citationSun, R., Jia, T., Dart, B., Shrestha, S., Bretches, M., Heggeness, M. H., & Yang, S. -. (2021). Human peripheral nerve-derived pluripotent cells can be stimulated by in vitro bone morphogenetic protein-2. Bioengineering, 8(10) doi:10.3390/bioengineering8100132en_US
dc.identifier.issn2306-5354
dc.identifier.urihttps://doi.org/10.3390/bioengineering8100132
dc.identifier.urihttps://soar.wichita.edu/handle/10057/22244
dc.descriptionCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_US
dc.description.abstractWe have recently identified a population of cells within the peripheral nerves of adult rodent animals (mice and rats) that can respond to Bone Morphogenetic Protein-2 (BMP-2) exposure or physical injury to rapidly proliferate. More importantly, these cells exhibited embryonic differentiation potentials that could be induced into osteoblastic and endothelial cells in vitro. The current study examined human nerve specimens to compare and characterize the cells after BMP-2 stimulation. Fresh pieces of human nerve tissue were minced and treated with either BMP-2 (750 ng/mL) or a PBS vehicle for 12 h at 37 °C, before being digested in 0.2% collagenase and 0.05% trypsin-EDTA. Isolated cells were cultured in a restrictive stem cell medium. Significantly more cells were obtained from the nerve pieces with the BMP-2 treatment in comparison with the PBS vehicle controls. Cell colonies started to form at Day 3. Expressions of the four transcription factors, namely, Klf4, c-Myc, Sox2, and Oct4, were confirmed at both the transcriptional and translational levels. The cells can be maintained in the stem cell culture medium for at least 6 weeks without changing their morphology. When the cells were transferred to a fibroblast growth medium, dispersed spindle-shaped motile cells were noted and became fibroblast activated protein-α (FAP) positive with immunocytochemistry staining. The data suggest that human peripheral nerve tissue also contains a population of cells that can respond to BMP-2 and express Klf4, Sox2, cMyc, and Oct4—the four transcription factors driving cell pluripotency. These cells are able to differentiate into FAP-positive fibroblasts. In summary, in human peripheral nerves also reside a population of quiescent cells with pluripotency potential that may be the same cells as rodent nerve-derived adult stem (NEDAPS) cells. It is proposed that these cells are possibly at the core of a previously unknown natural mechanism for healing an injury.en_US
dc.description.sponsorshipThis research was partially supported by a Rising Star grant from Kansas BioScience Authority, University of Kansas School of Medicine-Wichita Dean’s Level 1 funding, and a K-INBRE scholarship by the National Institutes of Health (P20 GM103418).en_US
dc.language.isoen_USen_US
dc.publisherMDPIen_US
dc.relation.ispartofseriesBioengineering;Vol. 8, Iss. 10
dc.subjectPluripotent stem cellsen_US
dc.subjectBMP-2en_US
dc.subjectPeripheral nerveen_US
dc.subjectHumanen_US
dc.titleHuman peripheral nerve-derived pluripotent cells can be stimulated by in vitro bone morphogenetic protein-2en_US
dc.typeArticleen_US
dc.rights.holderCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_US


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