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dc.contributor.authorYang, Shang-You
dc.contributor.authorGarcia, Elka S.
dc.contributor.authorXia, Will
dc.contributor.authorWang, Anyu
dc.identifier.citationYang, S. -., Garcia, E., Xia, W., & Wang, A. (2021). Effects of hypoxia on proliferation and apoptosis of osteosarcoma cells. Anticancer Research, 41(10), 4781-4787. doi:10.21873/anticanres.15293en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractBackground: Hypoxia can happen during solid tumor growth including osteosarcoma. This study investigated the relationship of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) on osteosarcoma cell growth and apoptosis under hypoxic conditions. Materials and Methods: Human osteosarcoma cells were cultured under normal or hypoxic conditions. Inhibitors of HIF-1α and VEGF were applied to the cells separately or in combination to block the respective proteins. Cell proliferation and apoptosis were examined by MTT and TUNEL assays, and real-time PCR and ELISA were performed for mRNA and protein expression. Results: There was a dramatic decrease of cell proliferation and an elevation of apoptosis under hypoxia. Blockage of HIF-1α and VEGFR enhanced the cell growth retardation and promoted apoptotic changes. Moreover, blockage of HIF-1α significantly eliminated the expression of VEGF in the cell culture media, and vice versa. Conclusion: HIF-1α and VEGF work closely in regulating osteosarcoma cell growth under hypoxic conditions and blockage of either of them may subsequently influence the presence of the other.en_US
dc.description.sponsorshipThe Authors wish to thank Ms. Zheng Song for her excellent technical assistance. The majority of the data presented here are also included in one of the student authors - Elka Garcia's Master Thesis as part of a requirement for the Wichita State University Graduate School. This work was supported by research grants from Kansas Flossie E. West Memorial Foundation and Wichita Research & Medical Education Foundation.en_US
dc.publisherInternational Institute of Anticancer Researchen_US
dc.relation.ispartofseriesAnticancer Research;Vol. 41, Iss. 10
dc.titleEffects of hypoxia on proliferation and apoptosis of osteosarcoma cellsen_US
dc.rights.holderCopyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.en_US

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