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dc.contributor.authorBaird, Matthew A.
dc.contributor.authorShliaha, Pavel V.
dc.contributor.authorAnderson, Gordon A.
dc.contributor.authorMoskovets, Eugene
dc.contributor.authorLaiko, Victor
dc.contributor.authorMakarov, Alexander A.
dc.contributor.authorJensen, Ole N.
dc.contributor.authorShvartsburg, Alexandre A.
dc.date.accessioned2019-06-20T21:51:12Z
dc.date.available2019-06-20T21:51:12Z
dc.date.issued2019-04-29
dc.identifier.citationMatthew A. Baird, Pavel V. Shliaha, Gordon A. Anderson, Eugene Moskovets, Victor Laiko, Alexander A. Makarov, Ole N. Jensen, and Alexandre A. Shvartsburg. 2019. High-resolution differential ion mobility separations/orbitrap mass spectrometry without buffer gas limitations. Analytical Chemistry, 91 (10), 6918-6925en_US
dc.identifier.issn0003-2700
dc.identifier.otherWOS:000469304300071
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.9b01309
dc.identifier.urihttp://hdl.handle.net/10057/16369
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractStrong orthogonality between differential ion mobility spectrometry (FAIMS) and mass spectrometry (MS) makes their hybrid a powerful approach to separate isomers and isobars. Harnessing that power depends on high resolution in both dimensions. The ultimate mass resolution and accuracy are delivered by Fourier Transform MS increasingly realized in Orbitrap MS, whereas FAIMS resolution is generally maximized by buffers rich in He or H-2 that elevate ion mobility and lead to prominent non-Blanc effects. However, turbomolecular pumps have lower efficiency for light gas molecules and their flow from the FAIMS stage complicates maintaining the ultrahigh vacuum (UHV) needed for Orbitrap operation. Here we address this challenge via two hardware modifications: (i) a differential pumping step between FAIMS and MS stages and (ii) reconfiguration of vacuum lines to isolate pumping of the high vacuum (HV) region. Either greatly ameliorates the pressure increases upon He or H-2 aspiration. This development enables free optimization of FAIMS carrier gas without concerns about MS performance, maximizing the utility and flexibility of FAIMS/MS platforms.en_US
dc.description.sponsorshipNSF CAREER (CHE-1552640), with P.S.'s stay at WSU funded by the Lundbeck Foundation and Danish Cancer Society.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofseriesAnalytical Chemistry;v.91:no.10
dc.subjectElectron-transfer dissociationen_US
dc.subjectPeptideen_US
dc.subjectSensiivityen_US
dc.subjectProteinsen_US
dc.subjectConformationen_US
dc.subjectHeliumen_US
dc.subjectPhosphorylationen_US
dc.subjectPetroleomicsen_US
dc.subjectPerformanceen_US
dc.subjectPhaseen_US
dc.titleHigh-resolution differential ion mobility separations/orbitrap mass spectrometry without buffer gas limitationsen_US
dc.typeArticleen_US
dc.rights.holder© 2019 American Chemical Societyen_US


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