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dc.contributor.authorBaird, Matthew A.
dc.contributor.authorAnderson, Gordon A.
dc.contributor.authorShliaha, Pavel V.
dc.contributor.authorJensen, Ole Noerregaard
dc.contributor.authorShvartsburg, Alexandre A.
dc.date.accessioned2019-02-08T03:48:57Z
dc.date.available2019-02-08T03:48:57Z
dc.date.issued2019-01-15
dc.identifier.citationBaird, Matthew A.; Anderson, Gordon A.; Shliaha, Pavel V.; Jensen, Ole Noerregaard; Shvartsburg, Alexandre A. Differential ion mobility separations/mass spectrometry with high resolution in both dimensions. Anal. Chem., 2019, 91 (2), pp 1479–1485en_US
dc.identifier.issn0003-2700
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.8b04518
dc.identifier.urihttp://hdl.handle.net/10057/15799
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractStrong orthogonality to mass spectrometry makes differential ion mobility spectrometry (FAIMS) a powerful tool for isomer separations. However, high FAIMS resolution has been achieved overall only with buffers rich in He or H2. That obstructed coupling to Fourier transform mass spectrometers operating under ultrahigh vacuum, but exceptional m/z resolution and accuracy of FTMS are indispensable for frontline biological and environmental applications. By raising the waveform amplitude to 6 kV, we enabled high FAIMS resolution using solely N2 and thus straightforward integration with any MS platform: here Orbitrap XL with the electron transfer dissociation (ETD) option. The initial evaluation for complete histone tails (50 residues) with diverse post-translational modifications on alternative sites demonstrates a broad capability to separate and confidently identify the PTM localization variants in the middle-down range.en_US
dc.description.sponsorshipNSF CAREER (CHE-1552640), Lundbeck Foundation, and Danish Cancer Society.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofseriesAnalytical Chemistry;v.91:no.2
dc.subjectDissociationen_US
dc.subjectMass spectrometryen_US
dc.titleDifferential ion mobility separations/mass spectrometry with high resolution in both dimensionsen_US
dc.typeArticleen_US
dc.rights.holder© 2018 American Chemical Society.en_US


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