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dc.contributor.authorKaphle, Pranita
dc.contributor.authorLi, Yongchao
dc.contributor.authorYao, Li
dc.identifier.citationKaphle P, Li Y, Yao L. The mechanical and pharmacological regulation of glioblastoma cell migration in 3D matrices. J Cell Physiol. 2019;234:3948–3960en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractThe invasion of glioblastoma is a complex process based on the interactions of tumor cells and the extracellular matrix. Tumors that are engineered using biomaterials are more physiologically relevant than a two-dimensional (2D) cell culture system. Matrix metalloproteinases and the plasminogen activator generated by tumor cells regulate a tumor’s invasive behavior. In this study, microtumors were fabricated by encapsulating U87 glioma cells in Type I collagen and then glioma cell migration in the collagen hydrogels was investigated. Crosslinking of collagen with 8S-StarPEG increased the hydrogel viscosity and reduced the tumor cell migration speed in the hydrogels. The higher migration speed corresponded to the increased gene expression of MMP-2, MMP-9, urokinase plasminogen activator (uPA), and tissue plasminogen activator (tPA) in glioma cells grown in non-crosslinked collagen hydrogels. Inhibitors of these molecules hindered U87 and A172 cell migration in collagen hydrogels. Aprotinin and tranexamic acid did not inhibit U87 and A172 migration on the culture dish. This study demonstrated the differential effect of pharmacologic molecules on tumor cell motility in either a 2D or three-dimensional culture environment.en_US
dc.description.sponsorshipNational Institute of General Medical Sciences, Grant/Award Number: P20 GM103418; Wichita State University; Wichita Medical Research and Education Foundation (WMREF); Flossie E. West Memorial Trusten_US
dc.relation.ispartofseriesJournal of Cellular Physiology;v.234:no.4
dc.titleThe mechanical and pharmacological regulation of glioblastoma cell migration in 3D matricesen_US
dc.rights.holder© 2018 Wiley Periodicals, Inc.en_US

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