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dc.contributor.authorPerera, Krishani Dinali
dc.contributor.authorGalasiti Kankanamalage, Anushka Chathuranga
dc.contributor.authorRathnayake, Athri D.
dc.contributor.authorHoneyfield, A.
dc.contributor.authorGroutas, William C.
dc.contributor.authorChang, Kyeong-Ok
dc.contributor.authorKim, Yunjeong
dc.identifier.citationPerera, K.D.; Galasiti Kankanamalage, Anushka Chathuranga; Rathnayake, Athri D.; Honeyfield, A.; Groutas, William C.; Chang, Kyeong-Ok; Kim, Yunjeong. 2018. Protease inhibitors broadly effective against feline, ferret and mink coronaviruses. Antiviral Research, vol. 160:pp 79-86en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractFerret and mink coronaviruses typically cause catarrhal diarrhea in ferrets and minks, respectively. In recent years, however, systemic fatal coronavirus infection has emerged in ferrets, which resembles feline infectious peritonitis (FIP) in cats. FIP is a highly fatal systemic disease caused by a virulent feline coronavirus infection in cats. Despite the importance of coronavirus infections in these animals, there are no effective commercial vaccines or antiviral drugs available for these infections. We have previously reported the efficacy of a protease inhibitor in cats with FIP, demonstrating that a virally encoded 3C-like protease (3CLpro) is a valid target for antiviral drug development for coronavirus infections. In this study, we extended our previous work on coronavirus inhibitors and investigated the structure-activity relationships of a focused library of protease inhibitors for ferret and mink 3CLpro. Using the fluorescence resonance energy transfer assay, we identified potent inhibitors broadly effective against feline, ferret and mink coronavirus 3CLpro. Multiple amino acid sequence analysis and modelling of 3CLpro of ferret and mink coronaviruses were conducted to probe the structural basis for these findings. The results of this study provide support for further research to develop broad-spectrum antiviral agents for multiple coronavirus infections. To the best of our knowledge, this is the first report on small molecule inhibitors of ferret and mink coronaviruses.en_US
dc.description.sponsorshipNational Institutes of Health ( R01AI130092 ) and Morris Animal Foundation , Denver, CO ( D14FE-012 and D16FE-512 ).en_US
dc.relation.ispartofseriesAntiviral Research;v.160
dc.subjectFerret coronavirusen_US
dc.subjectMink coronavirusen_US
dc.subjectFeline coronavirusen_US
dc.subject3C-like proteaseen_US
dc.subjectProtease inhibitoren_US
dc.titleProtease inhibitors broadly effective against feline, ferret and mink coronavirusesen_US
dc.rights.holder© 2018 Elsevier B.V. All rights reserved.en_US

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