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dc.contributor.authorYang, Shuye
dc.contributor.authorZhang, Kai
dc.contributor.authorJiang, Jianhao
dc.contributor.authorJames, Bonface
dc.contributor.authorYang, Shang-You
dc.identifier.citationYang S, Zhang K, Jiang J, James B, Yang S‐Y. 2018. Particulate and ion forms of cobalt–chromium challenged preosteoblasts promote osteoclastogenesis and osteolysis in a murine model of prosthesis failure. J Biomed Mater Res Part A. 2018:1–8en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractThis study investigated the interactive behavior of the particulate and ion forms of cobalt–chromium (Co–Cr) alloy challenged preosteoblasts during the process of prosthetic implant loosening. Preosteoblasts were challenged with Co–Cr particles or Co(II) ions for 72 h, followed by the proliferation and PCR assays. For in vivo test, a titanium pin was implanted into proximal tibia of SCID mice to mimic knee replacement. Co–Cr particles or Co(II) ion challenged preosteoblasts (5 × 105) were intra‐articularly injected into the implanted knee. The animals were sacrificed 5 weeks post‐op, and the prosthetic knees were harvested for biomechanical pin‐pullout testing, histological evaluations, and microCT assessment. In vitro study suggested that Co–Cr particles and Co(II) ions significantly suppressed the proliferation of preosteoblasts in a dose‐dependent manner. RT‐PCR data on the challenged cells indicated overexpression of receptor activator of nuclear factor kappa‐B ligand (RANKL) and inhibited osteoprotegerin (OPG) gene expression. Introduction of the differently challenged preosteoblasts to the pin‐implant mouse model resulted in reduced implant interfacial shear strength, thicker peri‐implant soft‐tissue formation, more TRAP+ cells, lower bone mineral density, and bone volume fraction. In conclusion, both Co–Cr particles and Co(II) ions interfered with the growth, maturation, and functions of preosteoblasts, and provides evidence that the metal ions as well play an important role in effecting preosteoblasts in the pathogenesis of aseptic loosening.en_US
dc.relation.ispartofseriesBiomedical Materials Research Part A;2018
dc.subjectAseptic looseningen_US
dc.subjectMetal ionen_US
dc.subjectWear debrisen_US
dc.titleParticulate and ion forms of cobalt–chromium challenged preosteoblasts promote osteoclastogenesis and osteolysis in a murine model of prosthesis failureen_US
dc.rights.holder© 2018 Wiley Periodicals, Inc.en_US

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