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dc.contributor.authorGong, Maojun
dc.contributor.authorZhang, Ning
dc.contributor.authorMaddukuri, Naveen
dc.identifier.citationM. Gong, N. Zhang and N. Maddukuri, Anal. Methods, 2018, 10, 3131en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractFlow-gated capillary electrophoresis (CE) is a hybrid of conventional and microchip CE since it employs a fused silica capillary as the separation channel while taking advantage of the rapid flow-gated injection that is mainly used in microchip CE. Compared with conventional CE, flow-gated CE has excellent flexibility to be combined with online sample pre-treatment procedures such as microdialysis, analyte derivatization, sample cleanup and enrichment via solid phase extraction, as well as 2D separation coupled with HPLC or CE. Additionally, the rapid sample injection may improve the separation efficiency and analytical throughput. Compared with electrophoresis on a microchip, flow-gated CE has advantages such as high separation efficiency, low capillary cost, convenient coupling with different detectors, and using fresh separation buffer. To build flow-gated CE systems, several configurations of flow-gating interfaces have been developed, out of which the transverse flow-gating configuration predominates with advantages such as small void volumes and easy preparation. Flow-gated CE has shown cutting-edge applications in in vivo measurements of neurotransmitters and in vitro monitoring of chemicals released by cultured cells or tissues. In addition, flow-gated CE, as the second dimensional separation method, has been successfully coupled with HPLC and CE to perform 2D separation. This review covers the basic instrumental setup and characteristics of flow-gated CE, flow-gating interfaces, and major applications, as well as coupling with various analytical techniques. Finally, a brief statement about the future direction of flow-gated CE is discussed. The review aims to provide the scientific community with meaningful knowledge and progress about flow-gated CE thus promoting its further development and applications.en_US
dc.description.sponsorshipNational Institute of General Medical Sciences with the grant number P20GM103418.en_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofseriesAnalytical Methods;v.10:no.26
dc.subjectSolid-phase extractionen_US
dc.subjectLaser-induced fluorescenceen_US
dc.subjectPush-pull perfusionen_US
dc.subjectComprehensive 2-dimensional systemen_US
dc.subjectAmino-acid neurotransmittersen_US
dc.subjectSampling in-vivoen_US
dc.subjectGating interfaceen_US
dc.subjectMicrochip electrophoresisen_US
dc.titleFlow-gated capillary electrophoresis: a powerful technique for rapid and efficient chemical separationen_US
dc.rights.holder© Royal Society of Chemistry 2018.en_US

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