Browsing BIO Faculty Scholarship by Author "Ward, Darrell N."

Now showing items 1-5 of 5

    • Circular dichroic spectroscopy of Arg46-nicked ovine lutropin α and derived fragments 

      Peng, Kou-Cheng; Bousfield, George R.; Puett, David; Ward, Darrell N. (Kluwer Academic Publishers-Plenum Publishers, 1996-08)
      Theα subunit of ovine lutropin can be nicked with the endoproteinase Arg-C to give a single cleavage of the Arg46-Ser47 peptide bond. Following reduction by sulfitolysis, the N-terminal (residues 1–46) and C-terminal ...

    • Evidence for two folding domains in glycoprotein hormone alpha-subunits 

      Bousfield, George R.; Ward, Darrell N. (The Endocrine Society, 1994-08)
      We reconstituted ovine (o) LH alpha from its amino- and carboxyl-terminal fragments obtained as follows. oLH alpha was nicked at Arg46-Ser47 with Arg-C protease. Nicked oLH alpha disulfide bonds were broken by sulfitolysis, ...

    • Initial characterization of equine inhibin 

      Moore, Katherine H.; Dunbar, Bonnie S.; Bousfield, George R.; Ward, Darrell N. (Society for the Study of Reproduction, 1994-07-01)
      Inhibin has been characterized from a number of mammals; however, it has not been extensively studied in horses. Western blot analysis was used to examine the size heterogeneity of equine inhibin a- and [S-subunits. The ...

    • Reduction and reoxidation of equine gonadotropin alpha-subunits 

      Bousfield, George R.; Ward, Darrell N. (The Endocrine Society, 1992-12)
      Ovine (o) and equine (e) LH alpha-subunits were reduced and reoxidized using conditions known to be effective for bovine and human alpha-subunits. The major product of oLH alpha refolding was alpha-subunit monomer. In ...

    • Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits 

      Matsui, Taei; Mizuochi, Tsuguo; Titani, Koiti; Okinaga, Tatsuyuki; Hoshi, Motonori; Bousfield, George R.; Sugino, Hiromu; Ward, Darrell N. (American Chemical Society, 1994-11)
      Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of a- and P-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between ...