Browsing BIO Faculty Scholarship by Author "Harvey, David J."
Now showing items 1-9 of 9
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All-or-none N-glycosylation in primate follicle-stimulating hormone beta-subunits
Bousfield, George R.; Butnev, Vladimir Y.; Walton, Wendy J.; Nguyen, Van T.; Huneidi, Jennifer; Singh, Vinod; Kolli, V. S. Kumar; Harvey, David J.; Rance, Naomi E. (Elsevier Ireland Ltd, 2007-01-02)Human FSH exists as two major glycoforms designated, tetra-glycosylated and di-glycosylated hFSH. The former possesses both alpha- and beta-subunit carbohydrates while the latter possesses only alpha-subunit carbohydrate. ... -
Differential effects of alpha subunit Asparagine56 oligosaccharide structure on equine lutropin and follitropin hybrid conformation and receptor-binding activity
Bousfield, George R.; Butnev, Vladimir Y.; Butnev, Viktor Y.; Nguyen, Van T.; Gray, Ciann M.; Dias, James A.; MacColl, Robert; Eisele, Leslie; Harvey, David J. (American Chemical Society, 2004-08-24)The gonadotropins, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and chorionic gonadotropin (CG), are cysteine-knot growth-factor superfamily glycoproteins composed of a common alpha subunit noncovalently ... -
Differentiation between sulphated and phosphated carbohydrates in low-resolution matrix-assisted laser desorption/ionization mass spectra
Harvey, David J.; Bousfield, George R. (John Wiley & Sons, Ltd., 2005-01-30)Differentiation between sulphated and phosphated carbohydrates in low-resolution matrix-assisted laser desorption/ionization mass spectra -
FGDB: Database of follicle stimulating hormone glycans
Shakyawar, Sushil K.; Pandey, Sanjit; Harvey, David J.; Bousfield, George R.; Guda, Chittibabu (Elsevier, 2021)Glycomics, the study of the entire complement of sugars of an organism has received significant attention in the recent past due to the advances made in high throughput mass spectrometry technologies. These analytical ... -
Follicle-stimulating hormone glycobiology
Bousfield, George R.; Harvey, David J. (NLM (Medline), 2019-06-01)FSH glycosylation varies in two functionally important aspects: microheterogeneity, resulting from oligosaccharide structure variation, and macroheterogeneity, arising from partial FSHβ subunit glycosylation. Although ... -
Human FSH glycoform α-Subunit asparagine$^{52}$ glycans: Major glycan structural consistency, minor glycan variation in abundance
Butnev, Viktor Y.; May, Jeffrey V.; Brown, Alan R.; Sharma, Tarak; Butnev, Vladimir Y.; White, William K.; Harvey, David J.; Bousfield, George R. (Frontiers Media S.A., 2022-10-18)Follicle-stimulating hormone (FSH), an α/β heterodimeric glycoprotein hormone, consists of functionally significant variants resulting from the presence or absence of either one of two FSHβ subunit N-glycans. The two most ... -
Hypo-glycosylated hFSH binds and activates FSH receptors more rapidly than fully-glycosylated hFSH
Bousfield, George R.; Butnev, Vladimir Y.; May, Jeffrey V.; Harvey, David J.; Jiang, Chao; Davis, John S. (FASEB: Federation of American Societies for Experimental Biology, 2013-04)Follitropin (FSH) heterodimers possess two potential N-glycosylation sites on both common α and hormone-specific β subunits. Hypo-glycosylated hFSH isolated from lutropin preparations possessed a partially glycosylated ... -
Hypo-glycosylated human follicle-stimulating hormone (hFSH(21/18)) is much more active in vitro than fully-glycosylated hFSH (hFSH(24))
Bousfield, George R.; Butnev, Vladimir Y.; Butnev, Viktor Y.; Hiromasa, Yasuaki; Harvey, David J.; May, Jeffrey V. (Elsevier Ireland Ltd, 2014-02-15)Hypo-glycosylated hFSH21/18 (possesses FSHβ21 and FSHβ18bands) was isolated from hLH preparations by immunoaffinity chromatography followed by gel filtration. Fully-glycosylated hFSH24 was prepared by combining the ... -
Production, purification, and characterization of recombinant hFSH glycoforms for functional studies
Butnev, Viktor Y.; Butnev, Vladimir Y.; May, Jeffrey V.; Shuai, Bin; Tran, Patrick; White, William K.; Brown, Alan; Hall, Aaron Smalter; Harvey, David J.; Bousfield, George R. (Elsevier Ireland Ltd., 2015-04-15)Previously, our laboratory demonstrated the existence of a beta-subunit glycosylation-deficient human FSH glycoform, hFSH(21). A third variant, hFSH(18), has recently been detected in FSH glycoforms isolated from purified ...