Now showing items 1-5 of 5

    • Bioactivity of recombinant hFSH glycosylation variants in primary cultures of porcine granulosa cells 

      Liang, Aixin; Plewes, Michele R.; Hua, Guohua; Hou, Xiaoying; Blum, Haley R.; Przygrodzka, Emilia; George, Jitu W.; Clark, Kendra L.; Bousfield, George R.; Butnev, Viktor Y.; May, Jeffrey V.; Davis, John S. (Elsevier, 2020-06-15)
      Previous studies have reported hypo-glycosylated FSH and fully-glycosylated FSH to be naturally occurring in humans, and these glycoforms exist in changing ratios over a woman's lifespan. The precise cellular and molecular ...
    • Hypo-glycosylated hFSH binds and activates FSH receptors more rapidly than fully-glycosylated hFSH 

      Bousfield, George R.; Butnev, Vladimir Y.; May, Jeffrey V.; Harvey, David J.; Jiang, Chao; Davis, John S. (FASEB: Federation of American Societies for Experimental Biology, 2013-04)
      Follitropin (FSH) heterodimers possess two potential N-glycosylation sites on both common α and hormone-specific β subunits. Hypo-glycosylated hFSH isolated from lutropin preparations possessed a partially glycosylated ...
    • Hypoglycosylated hFSH has greater bioactivity than fully glycosylated recombinant hFSH in human granulosa cells 

      Jiang, Chao; Hou, Xiaoying; Wang, Cheng; May, Jeffrey V.; Butnev, Viktor Y.; Bousfield, George R.; Davis, John S. (Endocrine Society, 2015-06)
      Context: Previous studies suggest that aging in women is associated with a reduction in hypoglycosylated forms of FSH. Objective: Experiments were performed to determine whether glycosylation of the FSH beta subunit modulates ...
    • In vivo and in vitro impact of carbohydrate variation on human follicle-stimulating hormone function 

      Bousfield, George R.; May, Jeffrey V.; Davis, John S.; Dias, James A.; Kumar, T. Rajendra (Frontiers Media S.A., 2018-05-09)
      Human follicle-stimulating hormone (FSH) exhibits both macro-and microheterogeneity in its carbohydrate moieties. Macroheterogeneity results in three physiologically relevant FSH beta subunit variants, two that possess a ...
    • Inositol phosphate stimulation by LH requires the entire alpha Asn56 oligosaccharide 

      Nguyen, Van T.; Singh, Vinod; Butnev, Vladimir Y.; Gray, Ciann M.; Westfall, Suzanne; Davis, John S.; Dias, James A.; Bousfield, George R. (Elsevier Ireland Ltd, 2003-01-31)
      Lentil lectin-bound, fucose-enriched hTSH was reported to stimulate both cAMP and inositol phosphate (IP) intracellular signalling pathways, whereas fucose-depleted hTSH stimulated only the cAMP pathway. Gonadotropins ...