Browsing BIO Faculty Scholarship by Author "Baker, Vanda L."
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Carbohydrate analysis of glycoprotein hormones
Bousfield, George R.; Baker, Vanda L.; Gotschall, R. Russell; Butnev, Viktor Y. (Academic Press, 2000-05)Complete carbohydrate composition analysis of glycoprotein hormones, their subunits, and oligosaccharides isolated from individual glycosylation sites can be accomplished using high-pH anion-exchange chromatography combined ... -
Glycosylated equine prolactin and its carbohydrate moiety
Butnev, Viktor Y.; Gotschall, R. Russell; Baker, Vanda L.; Moore, William T.; Gout, Peter W.; Bousfield, George R. (Springer New YorkSpringer New York, 1996-07)Glycosylated equine prolactin (G-ePRL) and nonglycosylated ePRL were purified to homogeneity from side fractions obtained during isolation of LH/FSH from horse pituitaries. Both PRL forms were isolated together in high ... -
Hormone-specific inhibitory influence of alpha-subunit Asn56 oligosaccharide on in vitro subunit association and follicle-stimulating hormone receptor binding of equine gonadotropins
Butnev, Viktor Y.; Gotschall, R. Russell; Butnev, Vladimir Y.; Baker, Vanda L.; Moore, William T.; Bousfield, George R. (Society for the Study of Reproduction, 1998-02-01)Hybrid hormones were created using combinations of equine (e) LH, eFSH, and eCG alpha- and beta-subunit preparations. The efficiency of eFSH beta association was highest with eLH alpha (64-72%) and was lowest with eCG alpha ... -
Negative influence of O-linked oligosaccharides of high molecular weight equine chorionic gonadotropin on its luteinizing hormone and follicle-stimulating hormone receptor-binding activities
Butnev, Viktor Y.; Gotschall, R. Russell; Baker, Vanda L.; Moore, William T.; Bousfield, George R. (The Endocrine Society, 1996-06)Three equine CG (eCG) forms with identical amino acid sequences but different mol wt and monosaccharide compositions were isolated from a crude eCG preparation and designated eCG-L (low mol wt), eCG-M (medium mol wt), and ... -
Structural features of mammalian gonadotropins
Bousfield, George R.; Butnev, Viktor Y.; Gotschall, R. Russell; Baker, Vanda L.; Moore, William T. (Elsevier Ireland, 1996-12-20)There are two species for which both pituitary and placental gonadotropins are readily available, humans and horses. The human gonadotropins are better characterized than equine gonadotropins. Nevertheless, the latter are ...