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dc.contributor.authorPadmanabhan, Ramesh
dc.contributor.authorHendry, Isabel R.
dc.contributor.authorKnapp, Jennifer R.
dc.contributor.authorShuai, Bin
dc.contributor.authorHendry, William J. III
dc.date.accessioned2017-09-17T18:11:01Z
dc.date.available2017-09-17T18:11:01Z
dc.date.issued2017-10
dc.identifier.citationPadmanabhan, R., Hendry, I.R., Knapp, J.R. et al. Cell Biol Toxicol (2017) 33: 483en_US
dc.identifier.issn0742-2091
dc.identifier.otherWOS:000409117700005
dc.identifier.urihttp://dx.doi.org/10.1007/s10565-017-9389-6
dc.identifier.urihttp://hdl.handle.net/10057/14084
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractTreatment of Syrian hamsters on the day of birth with the prototypical endocrine disruptor and synthetic estrogen, diethylstilbestrol (DES), leads to 100% occurrence of uterine hyperplasia/dysplasia in adulthood, a large proportion of which progress to neoplasia (endometrial adenocarcinoma). Consistent with our prior gene expression analyses at the mRNA and protein levels, we now report (based on microarray, real-time polymerase chain reaction, and in situ hybridization analyses) that progression of the neonatal DES-induced dysplasia/neoplasia phenomenon in the hamster uterus also includes a spectrum of microRNA expression alterations (at both the whole-organ and cell-specific level) that differ during the initiation (upregulated miR-21, 200a, 200b, 200c, 29a, 29b, 429, 141; downregulated miR-181a) and promotion (downregulated miR-133a) stages of the phenomenon. The biological processes targeted by those differentially expressed miRNAs include pathways in cancer and adherens junction, plus regulation of the cell cycle, apoptosis, and miRNA functions, all of which are consistent with our model system phenotype. These findings underscore the need for continued efforts to identify and assess both the classical genetic and the more recently recognized epigenetic mechanisms that truly drive this and other endocrine disruption phenomena.en_US
dc.description.sponsorshipFlossie E. West Foundation, by the KIDDRC grant NICHD HD 002528, and by Grants #P20 RR016475 from the National Center for Research Resources and #P20 GM103418 from the National Institute of General Medical Sciences.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Netherlandsen_US
dc.relation.ispartofseriesCell Biology and Toxicology;v.33:no.5
dc.subjectDiethylstilbestrolen_US
dc.subjectEndocrine disruptionen_US
dc.subjectFemale reproductive systemen_US
dc.subjectMicroRNAen_US
dc.subjectNeoplasiaen_US
dc.subjectUterusen_US
dc.titleAltered microRNA expression patterns during the initiation and promotion stages of neonatal diethylstilbestrol-induced dysplasia/neoplasia in the hamster (Mesocricetus auratus) uterusen_US
dc.typeArticleen_US
dc.rights.holderCopyright © 2017, Springer Science+Business Media Dordrechten_US


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