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dc.contributor.authorBerndt, Marcus
dc.contributor.authorLi, Yongchao
dc.contributor.authorSeyedhassantehrani, Negar
dc.contributor.authorYao, Li
dc.identifier.citationMarcus Berndt, Yongchao Li, Negar Seyedhassantehrani, and Li Yao. 2017. Fabrication and characterization of microspheres encapsulating astrocytes for neural regeneration. ACS Biomater. Sci. Eng., 2017, 3 (7), pp 1313–1321en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractAstrocytes play a critical role in supporting the normal physiological function of neurons. Recent studies have revealed that astrocyte transplantation can promote axonal regeneration and functional recovery after spinal cord injury. Biomaterial can be designed as a growth-permissive substrate and serve as a carrier for astrocyte transplantation into injured spinal cord. In this study, we developed a method to generate collagen microspheres encapsulating astrocytes by injecting a mixture of collagen and astrocytes into a cell culture medium with a syringe controlled by a syringe pump. The collagen microspheres were cross-linked with poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-StarPEG) to reduce the degradation rate. The viability of cells in the cross-linked microspheres was higher than 90%. Astrocytes were transfected with plasmids encoding nerve growth factor (NGF)-ires-enhanced green fluorescent protein (EGFP) genes by electroporation and encapsulated in cross-linked microspheres. The level of NGF released into the cell culture medium was higher than that remaining in the microspheres or astrocytes. When microspheres encapsulating astrocytes transfected with plasmids encoding NGF-ires-EGFP genes were added into the cultured rat dorsal root ganglion, the axonal growth was significantly enhanced. This study shows that the microspheres can be potentially used as a carrier of astrocytes to promote nerve regeneration in injured neural tissue.en_US
dc.description.sponsorshipWichita State University, and the National Institute of General Medical Sciences (P20 GM103418) of the National Institutes of Health.en_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofseriesACS Biomaterials Science & Engineering;v.3:no.7
dc.subjectNeurite growthen_US
dc.titleFabrication and characterization of microspheres encapsulating astrocytes for neural regenerationen_US
dc.rights.holderCopyright © 2016 American Chemical Societyen_US

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