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dc.contributor.authorBowman, Andrew P.
dc.contributor.authorAbzalimov, Rinat R.
dc.contributor.authorShvartsburg, Alexandre A.
dc.identifier.citationBowman, Andrew P.; Abzalimov, Rinat R.; Shvartsburg, Alexandre A. 2017. Broad separation of isomeric lipids by high-resolution differential ion mobility spectrometry with tandem mass spectrometry. Journal of The American Society for Mass Spectrometry, vol. 28:no. 8:pp 1552–1561en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractMaturation of metabolomics has brought a deeper appreciation for the importance of isomeric identity of lipids to their biological role, mirroring that for proteoforms in proteomics. However, full characterization of the lipid isomerism has been thwarted by paucity of rapid and effective analytical tools. A novel approach is ion mobility spectrometry (IMS) and particularly differential or field asymmetric waveform IMS (FAIMS) at high electric fields, which is more orthogonal to mass spectrometry. Here we broadly explore the power of FAIMS to separate lipid isomers, and find a similar to 75% success rate across the four major types of glycero- and phospho-lipids (sn, chain length, double bond position, and cis/trans). The resolved isomers were identified using standards, and (for the first two types) tandem mass spectrometry. These results demonstrate the general merit of incorporating high-resolution FAIMS into lipidomic analyses.en_US
dc.description.sponsorshipNIH K-INBRE (P20 GM103418), NSF First (EPS-0903806), and NSF CAREER (CHE-1552640).en_US
dc.relation.ispartofseriesJournal of The American Society for Mass Spectrometry;v.28:no.8
dc.subjectIon mobility spectrometryen_US
dc.titleBroad separation of isomeric lipids by high-resolution differential ion mobility spectrometry with tandem mass spectrometryen_US
dc.rights.holderCopyright © 2017, American Society for Mass Spectrometryen_US

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