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    Enantiomer separations by capillary electrophoresis

    Date
    2016-09-20
    Author
    Scriba, Gerhard K. E.
    Harnisch, Henrik
    Zhu, Qingfu
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    Citation
    Scriba, Gerhard K. E.; Harnisch, Henrik; Zhu, Qingfu. 2016. Enantiomer separations by capillary electrophoresis. Capillary Electrophoresis, vol. 1483 of the series Methods in Molecular Biology:pp 277-299
    Abstract
    Capillary electrophoresis (CE) is a versatile and flexible technique for analytical enantioseparations. This is due to the large variety of chiral selectors as well as the different operation modes including electrokinetic chromatography, micellar electrokinetic chromatography, and microemulsion electrokinetic chromatography. The chiral selector, which is added to the background electrolyte, represents a pseudostationary phase with its own electrophoretic mobility allowing a variety of different separation protocols. The present chapter briefly addresses the basic fundamentals of CE enantioseparations as well as the most frequently applied chiral selectors and separation modes. The practical example illustrates the separation of the enantiomers of a positively charged analyte using native and charged cyclodextrin derivatives as chiral selectors.
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    URI
    http://dx.doi.org/10.1007/978-1-4939-6403-1_15
    http://hdl.handle.net/10057/13137
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