Differential regulatory gene expression at the proteomic level in subtypes of human ovarian cancer cell-lines
Hendry, Isabel R.
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Ovarian carcinoma is the most lethal neoplasm and the fifth leading cause of mortality in women. Among the different subtypes, widely heterogeneous high-grade serous ovarian cancer (HGSOC) poses a great challenge to modern chemotherapy, due to its high recurrence rate and resistance to standard treatments. Recent studies suggest that survival time-line and disease recurrence are linked to the variable expression of biomarkers in cancer cells. Therefore, using Western blot analyses, we assessed the expression of a host of regulatory gene protein products at the whole-cell level in extracts from both three HGSOC and five non-HGSOC cell lines. Some of our most distinctive findings from those analyses were that: 1) Nrf2, a key factor in the anti-oxidant response element system, is expressed as a series of protein bands of variable molecular weight in all but the Kuramochi (HGSOC) cell line; 2) Estrogen receptor alpha is expressed in all but the OVSAHO (HGSOC) cell line; and 3) the AUF-1, p120-catenin, and NFKB-p50 proteins are expressed in both HGSOC and non-HGSOC cell types. We will next follow up those findings conducted at the whole cell level with assessments at the individual cell and subcellular level using immunohistochemical analyses.
Poster project completed at Wichita State University, Department of Biological Sciences.
Presented at the 14th Annual Capitol Graduate Research Summit, Topeka, KS, March 03, 2017.