| dc.contributor | Wichita State University | |
| dc.contributor.author | Bann, James G. | |
| dc.date.accessioned | 2016-11-08T02:08:47Z | |
| dc.date.available | 2016-11-08T02:08:47Z | |
| dc.date.issued | 2010-06-08 | |
| dc.date.submitted | 2007-11-13 | |
| dc.identifier | US 7,731,979 B2 | |
| dc.identifier.citation | Bann, James G., inventor. Wichita State University, assignee. Protective antigen having fluorinated histidine residues. Unites States patent: US 7,731,979. 2010, Jun 8. | |
| dc.identifier.uri | http://hdl.handle.net/10057/12561 | |
| dc.identifier.uri | https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7731979 | |
| dc.description | WSU inventor: James G. Bann, Dept. of Chemistry, College of Liberal Arts and Sciences | |
| dc.description | Appl. no.: 11/939,249 filed Nov. 13, 2007. U.S. Patent 7,731,979 granted Jun 8, 2010. | |
| dc.description.abstract | The unnatural amino acid analogue 2-fluorohistidine (2-FHis) was incorporated into protective antigen to produce a protein which resists protonation at physiological pH by reducing the side-chain pKa. The protein structure was unperturbed by the incorporation of fluorinated histidine residues, and the heptameric (2-FHisPA.sub.63).sub.7 could form ion conducting channels, and bind to the PA-binding domain of LF (LF.sub.N), but translocation of LF.sub.N in planar lipid bilayers was blocked. Further, while (2-FHisPA.sub.63).sub.7 could bind to host cells and in vitro to the host cellular receptor, pore formation in the presence of the receptor was blocked, and LF.sub.N-DTA mediated cytotoxicity in CHO-K1 cells was blocked. The modified PA is useful as both a vaccine and an antitoxin, providing epitopes for the production of antibodies against PA, but preventing key steps in pathogenesis (pore formation, translocation). | |
| dc.format.extent | 6 claims; 12 drawing sheets; 27 pages. | |
| dc.language | en_US | |
| dc.publisher | United States Patent and Trademark Office | |
| dc.rights | Status: Active;
Adjusted expiration: 2027-12-01 | |
| dc.subject.classification | USPC.: 424/246.1 | |
| dc.subject.classification | CPC: C07K 14/32 (20130101); A61K 39/00 (20130101) | |
| dc.subject.classification | IPC.: A61K 39/07 (20060101); A61K 38/00 (20060101); A61K 39/02 (20060101); A61K 39/38 (20060101); A61K 51/00 (20060101) | |
| dc.title | Protective antigen having fluorinated histidine residues | |
| dc.type | Patent | |
