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dc.contributor.advisorShuai, Bin
dc.contributor.authorBhandari, Prajwal
dc.date.accessioned2016-07-06T14:58:46Z
dc.date.available2016-07-06T14:58:46Z
dc.date.issued2016-04-29
dc.identifier.citationBhandari, Prajwal. 2016. Developing a qPCR assay to quantify disease severity in Medicago truncatula due to Macrophomina phaseolina infection. --In Proceedings: 12th Annual Symposium on Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p. 26
dc.identifier.urihttp://hdl.handle.net/10057/12173
dc.descriptionPresented to the 12th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Heskett Center, Wichita State University, April 29, 2016.
dc.descriptionResearch completed at Department of Biological Sciences, Fairmount College of Liberal Arts and Sciences
dc.description.abstractHaving host range over 500 crop species, the soil borne necrotrophic fungus Macrophomina phaseolina, is one of the catastrophic factors in current agricultural sector. It infects plant during stressful environmental conditions such as hot and dry weather, resulting in alteration of plant growth, yield, and seed quality. In order to establish proper disease control system and develop disease resistant cultivars, we have established a pathosystem in the model plant, Medicago truncatula to study the molecular interactions between the host and the pathogen. In this study, we proposed to establish an unbiased quantitative assay using quantitative PCR (qPCR), which is more objective than other previously used approaches such as, scoring system based on percentage of necrosis and chlorosis seen on aerial part of plants or colony count approach. qPCR can quantitate the amount of fungal and plant DNA during infection over time, which are then used to understand disease progression by comparing amount of DNA from two different sources. This assay also facilitates disease diagnosis/assessment in the field.
dc.description.sponsorshipGraduate School, Academic Affairs, University Libraries, Regional Institute on Aging
dc.language.isoen_US
dc.publisherWichita State University
dc.relation.ispartofseriesGRASP
dc.relation.ispartofseriesv. 12
dc.titleDeveloping a qPCR assay to quantify disease severity in Medicago truncatula due to Macrophomina phaseolina infection
dc.typeAbstract
dc.rights.holderWichita State University


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