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dc.contributor.authorGurung, Ritu
dc.contributor.authorYadav, Rahul
dc.contributor.authorBrungardt, Joseph G.
dc.contributor.authorOrlova, Albina
dc.contributor.authorEgelman, Edward H.
dc.contributor.authorBeck, Moriah R.
dc.identifier.citationRitu Gurung, Rahul Yadav, Joseph G. Brungardt, Albina Orlova, Edward H. Egelman, Moriah R. Beck. Actin polymerization is stimulated by actin cross-linking protein palladin. Biochemical Journal, Feb 2016, 473 (4) 383-396en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractThe actin scaffold protein palladin regulates both normal cell migration and invasive cell motility, processes that require the co-ordinated regulation of actin dynamics. However, the potential effect of palladin on actin dynamics has remained elusive. In the present study, we show that the actin-binding immunoglobulin-like domain of palladin, which is directly responsible for both actin binding and bundling, also stimulates actin polymerization in vitro. Palladin eliminated the lag phase that is characteristic of the slow nucleation step of actin polymerization. Furthermore, palladin dramatically reduced depolymerization, slightly enhanced the elongation rate, and did not alter the critical concentration. Microscopy and in vitro crosslinking assays reveal differences in actin bundle architecture when palladin is incubated with actin before or after polymerization. These results suggest a model whereby palladin stimulates a polymerization-competent form of globular or monomeric actin (G-actin), akin to metal ions, either through charge neutralization or through conformational changes.en_US
dc.description.sponsorshipNational Center for Research Resources [grant number 5P20RR017708], both a COBRE grant [grant number 8P20GM103420] and an Institutional Development Award (IDeA) [grant number P20GM103418] from the National Institute of General Medical Sciences at the National Institutes of Health, Burroughs-Wellcome Trust Collaborative Grant, the Flossie E. West Memorial Foundation Trust and by funds from Wichita State University.en_US
dc.publisherPortland Press Limiteden_US
dc.relation.ispartofseriesBiochemical Journal;v.473:no.4
dc.titleActin polymerization is stimulated by actin cross-linking protein palladinen_US
dc.rights.holder© 2016 Authors; published by Portland Press Limiteden_US

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