Show simple item record

dc.contributor.authorPriyadarshani, Priyanka
dc.contributor.authorLi, Yongchao
dc.contributor.authorYang, Shang-You
dc.contributor.authorYao, Li
dc.identifier.citationPriyadarshani P, Li Y, Yang SY, Yao L. 2016. Injectable hydrogel provides growth-permissive environment for human nucleus pulposus cells. J Biomed Mater Res Part A 2016:104A:419–426en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractDegeneration of intervertebral discs (IVDs) results in an overall alteration of the biomechanics of the spinal column and becomes a major cause of low back pain. In this study, an injectable hydrogel composite is fabricated and characterized as a potential scaffold for the treatment of degenerated IVDs. Crosslinking of type II collagen-hyaluronic acid (HA) hydrogel with 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) increases the gel stability against collagenase digestion and reduces water uptake in comparison with non-crosslinked gel. Cell viability assay exhibits the proliferation of human nucleus pulposus (HNP) cells in hydrogels. The cells in non-crosslinked gel and the gel crosslinked with a low concentration of EDC (0.1 mM) show superior cell viability and morphology compared with cells in gels crosslinked with higher concentration of EDC. Quantitative PCR assay demonstrates the gene expression of extracellular matrix (ECM) by cells cultured in the gels. The expression of ECM genes by HNP cells in the gels demonstrated the phenotypic change of the cells. This study suggests that the type II collagen-HA hydrogel and crosslinked hydrogel (0.1 mM EDC) are permissive matrix for the growth of HNP cells and can be potentially applied in NP repair.en_US
dc.description.sponsorshipContract grant sponsor: Wichita State University, the National Institute of General Medical Sciences; contract grant number: P20 GM103418 Contract grant sponsor: National Institutes of Health, and Wichita Medical Research & Education Foundation (WMREF) (NP cell isolation)en_US
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.relation.ispartofseriesJournal of Biomedical Materials Research Part A;v.104:no.2
dc.subjectNucleus pulposus cellsen_US
dc.subjectType II collagenen_US
dc.subjectHyaluronic aciden_US
dc.titleInjectable hydrogel provides growth-permissive environment for human nucleus pulposus cellsen_US
dc.rights.holder© 2015 Wiley Periodicals, Inc.en_US

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record