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dc.contributor.authorZhang, Qiyang
dc.contributor.authorMaddukuri, Naveen
dc.contributor.authorGong, Maojun
dc.identifier.citationZhang, Qiyang; Maddukuri, Naveen; Gong, Maojun. 2015. A direct and rapid method to determine cyanide in urine by capillary electrophoresis. Journal of Chromatography A, vol. 1414, 2 October 2015:pp 158–162en_US
dc.descriptionClick on the DOI link to access the article *may not be free).en_US
dc.description.abstractCyanides are poisonous chemicals that widely exist in nature and industrial processes as well as accidental fires. Rapid and accurate determination of cyanide exposure would facilitate forensic investigation, medical diagnosis, and chronic cyanide monitoring. Here, a rapid and direct method was developed for the determination of cyanide ions in urinary samples. This technique was based on an integrated capillary electrophoresis system coupled with laser-induced fluorescence (LIF) detection. Cyanide ions were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) and a primary amine (glycine) for LIF detection. Three separate reagents, NDA, glycine, and cyanide sample, were mixed online, which secured uniform conditions between samples for cyanide derivatization and reduced the risk of precipitation formation of mixtures. Conditions were optimized; the derivatization was completed in 2-4min, and the separation was observed in 25s. The limit of detection (LOD) was 4.0 nM at 3-fold signal-to-noise ratio for standard cyanide in buffer. The cyanide levels in urine samples from smokers and non-smokers were determined by using the method of standard addition, which demonstrated significant difference of cyanide levels in urinary samples from the two groups of people. The developed method was rapid and accurate, and is anticipated to be applicable to cyanide detection in waste water with appropriate modification.en_US
dc.description.sponsorshipNational Institute of General Medical Sciences with the grant number of P20 GM103418.en_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofseriesJournal of Chromatography A;v.1414
dc.subjectCapillary electrophoresisen_US
dc.titleA direct and rapid method to determine cyanide in urine by capillary electrophoresisen_US
dc.rights.holderCopyright © 2015 Elsevier B.V. or its licensors or contributors.en_US

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