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dc.contributor.authorCohen, Limor
dc.contributor.authorBousfield, George R.
dc.contributor.authorBen-Menahem, David
dc.identifier.citationCohen, Limor; Bousfield, George R.; Ben-Menahem, David. 2015. The recombinant equine LH beta subunit combines divergent intracellular traits of human LH beta and CG beta subunits. Theriogenology, vol. 83:no. 9, June 2015:pp 1469–1476en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractThe pituitary LH beta and placental CG beta subunits are products of different genes in primates. The major structural difference between the two subunits is in the carboxy-terminal region, where the short carboxyl sequence of hLH beta is replaced by a longer O-glycosylated carboxyterminal peptide in hCG beta. In association with this structural deviation, there are marked differences in the secretion kinetics and polarized routing of the two subunits. In equids, however, the CG beta and LH beta subunits are products of the same gene expressed in the placenta and pituitary (LH beta), and both contain a carboxy-terminal peptide. This unusual expression pattern intrigued us and led to our study of eLH beta subunit secretion by transfected Chinese hamster ovary and Mad in-Darby canine kidney cells. In continuous labeling and pulse-chase experiments, the secretion of the eLH beta subunit from the transfected Chinese hamster ovary cells was inefficient (medium recovery of 16%-25%) and slow (t(1/2)>6.5 hours). This indicated that, the secretion of the eLH beta subunit resembles that of hLH beta rather than hCG beta. In Madin-Darby canine kidney cells grown on Transwell filters, the eLH beta subunit was preferentially secreted from the apical side, similar to the hCG beta subunit secretory route (similar to 65% of the total protein secreted). Taken together, these data suggested that secretion of the eLH beta subunit integrates features of both hLH beta and hCG beta subunits. We propose that the evolution of this intracellular behavior may fulfill the physiological demands for biosynthesis of the LH and CG beta-subunits in the pituitary and placenta, respectively.en_US
dc.description.sponsorshipThis work was supported by grants from the Israel Science Foundation (to David Ben-Menahem; 448/03 and 1240/10).en_US
dc.publisherElsevier B.V.en_US
dc.subjectLH betaen_US
dc.subjectCG betaen_US
dc.subjectCarboxyl-terminal peptideen_US
dc.titleThe recombinant equine LH beta subunit combines divergent intracellular traits of human LH beta and CG beta subunitsen_US
dc.rights.holder© 2015 Elsevier Inc.

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