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dc.contributor.authorYao, Li
dc.contributor.authorLi, Yongchao
dc.contributor.authorKnapp, Jennifer R.
dc.contributor.authorSmith, Peter
dc.date.accessioned2015-04-24T21:57:51Z
dc.date.available2015-04-24T21:57:51Z
dc.date.issued2015-07
dc.identifier.citationYao, L., Li, Y., Knapp, J. and Smith, P. (2015), Exploration of molecular pathways mediating electric field-directed schwann cell migration by RNA-seq. J. Cell. Physiol., 230: 1515–1524. doi: 10.1002/jcp.24897en_US
dc.identifier.issn0021-9541
dc.identifier.otherWOS:000351842500013
dc.identifier.urihttp://dx.doi.org/10.1002/jcp.24897
dc.identifier.urihttp://hdl.handle.net/10057/11243
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractIn peripheral nervous systems, Schwann cells wrap around axons of motor and sensory neurons to form the myelin sheath. Following spinal cord injury, Schwann cells regenerate and migrate to the lesion and are involved in the spinal cord regeneration process. Transplantation of Schwann cells into injured neural tissue results in enhanced spinal axonal regeneration. Effective directional migration of Schwann cells is critical in the neural regeneration process. In this study, we report that Schwann cells migrate anodally in an applied electric field (EF). The directedness and displacement of anodal migration increased significantly when the strength of the EF increased from 50mV/mm to 200mV/mm. The EF did not significantly affect the cell migration speed. To explore the genes and signaling pathways that regulate cell migration in EFs, we performed a comparative analysis of differential gene expression between cells stimulated with an EF (100mV/mm) and those without using next-generation RNA sequencing, verified by RT-qPCR. Based on the cut-off criteria (FC>1.2, q<0.05), we identified 1,045 up-regulated and 1,636 down-regulated genes in control cells versus EF-stimulated cells. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found that compared to the control group, 21 pathways are down-regulated, while 10 pathways are up-regulated. Differentially expressed genes participate in multiple cellular signaling pathways involved in the regulation of cell migration, including pathways of regulation of actin cytoskeleton, focal adhesion, and PI3K-Akt. J. Cell. Physiol. 230: 1515-1524, 2015.en_US
dc.description.sponsorshipContract grant sponsor: Wichita State University. Contract grant sponsor: The Kansas Intellectual and Developmental Disabilities Research Center, the National Institute of General Medical Sciences; Contract grant number: P20 GM103418. Contract grant sponsor: Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health; Contract grant number: P20 GM103418.en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.relation.ispartofseriesJournal of Cellular Physiology;v.230:no.7
dc.subjectSpinal-cord-injuryen_US
dc.subjectActivated protein-kinaseen_US
dc.subjectExpression analysisen_US
dc.subjectEpithelial-cellsen_US
dc.subjectMotilityen_US
dc.subjectMyelinationen_US
dc.subjectIqgap1en_US
dc.subjectKeratinocytesen_US
dc.subjectChemotaxisen_US
dc.subjectReceptorsen_US
dc.titleExploration of molecular pathways mediating electric field-directed Schwann cell migration by RNA-Seqen_US
dc.typeArticleen_US
dc.rights.holderCopyright Wiley Periodicals 2015


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