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dc.contributor.authorHendry, William J. III
dc.contributor.authorHariri, Hussam Y.
dc.contributor.authorAlwis, Imala D.
dc.contributor.authorGunewardena, Sumedha S.
dc.contributor.authorHendry, Isabel R.
dc.date.accessioned2015-01-27T00:48:43Z
dc.date.available2015-01-27T00:48:43Z
dc.date.issued2014-12
dc.identifier.citationHendry, William J. III; Hariri, Hussam Y.; Alwis, Imala D.; Gunewardena, Sumedha S.; Hendry, Isabel R. 2014. Altered gene expression patterns during the initiation and promotion stages of neonatally diethylstilbestrol-induced hyperplasia/dysplasia/neoplasia in the hamster uterus. Reproductive Toxicology, vol. 50:pp 68–86en_US
dc.identifier.issn0890-6238
dc.identifier.otherWOS:000346459900010
dc.identifier.urihttp://dx.doi.org/10.1016/j.reprotox.2014.09.002
dc.identifier.urihttp://hdl.handle.net/10057/11053
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractNeonatal treatment of hamsters with diethylstilbestrol (DES) induces uterine hyperplasia/dysplasia/neoplasia (endometrial adenocarcinoma) in adult animals. We subsequently determined that the neonatal DES exposure event directly and permanently disrupts the developing hamster uterus (initiation stage) so that it responds abnormally when it is stimulated with estrogen in adulthood (promotion stage). To identify candidate molecular elements involved in progression of the disruption/neoplastic process, we performed: (1) immunoblot analyses and (2) microarray profiling (Affymetrix Gene Chip System) on sets of uterine protein and RNA extracts, respectively, and (3) immunohistochemical analysis on uterine sections; all from both initiation stage and promotion stage groups of animals. Here we report that: (1) progression of the neonatal DES-induced hyperplasia/dysplasia/neoplasia phenomenon in the hamster uterus involves a wide spectrum of specific gene expression alterations and (2) the gene products involved and their manner of altered expression differ dramatically during the initiation vs. promotion stages of the phenomenon.en_US
dc.description.sponsorshipFlossie E. West Foundation and by United States Public Health Service grants R15 HD37835, R21ES12308, and Grants #P20 RR016475 from the National Center for Research Resources, and #P20 GM103418 from the National Institute of General Medical Sciences.en_US
dc.language.isoen_USen_US
dc.publisherElsevier Incen_US
dc.relation.ispartofseriesReproductive Toxicology;v.50
dc.subjectFemale reproductive systemen_US
dc.subjectUterusen_US
dc.subjectDiethylstilbestrolen_US
dc.subjectEndocrine disruptionen_US
dc.subjectNeoplasiaen_US
dc.titleAltered gene expression patterns during the initiation and promotion stages of neonatally diethylstilbestrol-induced hyperplasia/dysplasia/neoplasia in the hamster uterusen_US
dc.typeArticleen_US
dc.rights.holderCopyright © 2014 Elsevier Inc. All rights reserved.


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