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dc.contributor.authorZhang, Kai
dc.contributor.authorYang, Shang-You
dc.contributor.authorYang, Shuye
dc.contributor.authorBai, Ling
dc.contributor.authorLi, Peng
dc.contributor.authorLiu, Dong
dc.contributor.authorSchurman, John R.
dc.contributor.authorWooley, Paul H.
dc.identifier.citationZhang, Kai; Yang, Shang-You; Yang, Shuye; Bai, Ling; Li, Peng; Liu, Dong; Schurman, John R.; Wooley, Paul H. 2015. Different influence of Ti, PMMA, UHMWPE, and Co-Cr particles on peripheral blood monocytes during periprosthetic inflammation. Journal of Biomedical Materials Research, Part A, vol. 103:no. 1:pp 358–364en_US
dc.descriptionClick on the DOI link to access the article (may not be free).en_US
dc.description.abstractThis study investigated cellular trafficking and inflammatory markers in orthopedic biomaterial particle-challenged human peripheral blood monocytes (PBMCs) using a murine immunodeficiency (SCID) model. Periprosthetic tissues from aseptic loosening patients were transplanted into muscles of SCID mice. PBMCs from the same patients were stimulated in vitro with Ti-6Al-4V, PMMA, UHMWPE, or Co-Cr particles for 3 days before administered intraperitoneally to the periprosthetic tissue-implanted mice. The xenografts were harvested 2 weeks later for histological and molecular analyses. Significant cell infiltration was obvious in the transplanted tissues from mice transfused with Ti-alloy, PMMA and UHMWPE-provoked PBMCs compared to controls, and UHMWPE-provoked PBMCs group accumulated significantly more cells among all groups. There were ubiquitous TRAP+ stained cells in all xenografts from particle-stimulated PBMCs mice. Immunohistochemical staining indicated that significantly more IL-1 and TNF positive cells occurred in Ti and PMMA groups; while the UHMWPE group resulted in stronger positive MCP-1 and IL-6 stains. Polymerase chain reaction (PCR) confirmed overexpression of both IL-1 and TNF in Ti and PMMA-stimulated groups; and more MIP-1 gene expression developed in the UHMWPE group. Overall, different type of orthopedic materials influenced the trafficking ability of particle-activated PBMCs which may depend on upregulation of various proinflammatory cytokines and chemokines.en_US
dc.description.sponsorshipContract grant sponsor: Orthopaedic Research & Education Foundation, USA Contract grant sponsor: National Natural Science Foundation of China; contract grant number: No. 81241061en_US
dc.publisherWiley Periodicals, Inc.en_US
dc.relation.ispartofseriesJournal of Biomedical Materials Research;v.103:no.1
dc.subjectAseptic looseningen_US
dc.subjectWear debrisen_US
dc.subjectCell traffickingen_US
dc.titleDifferent influence of Ti, PMMA, UHMWPE, and Co-Cr particles on peripheral blood monocytes during periprosthetic inflammationen_US
dc.rights.holder© 2014 Wiley Periodicals, Inc.

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