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    Genus-wide microsatellite primers for the Goldenrods (Solidago; Asteraceae)

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    Date
    2014-04
    Author
    Beck, James B.
    Semple, John C.
    Brull, Justin M.
    Lance, Stacey L.
    Phillips, Mai M.
    Hoot, Sara B.
    Meyer, Gretchen A.
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    Abstract
    Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago . • Methods and Results: Illumina sequencing of a shotgun library from S. gigantea identifi ed ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifi able, single-copy, and variable in a broad range of Solidago species. • Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecifi c studies in the ca. 120 Solidago species.
    Description
    © 2014 Beck et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
    URI
    http://dx.doi.org/10.3732/apps.1300093
    http://hdl.handle.net/10057/10717
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