Recovery and STR amplification of DNA from RFLP membranes
Steadman, Shelly A. McDonald, J. David Andrews, John S. Watson, Nigel D.
Steadman, Shelly A.
McDonald, J. David
Andrews, John S.
Watson, Nigel D.
Citations
Altmetric:
Other Names
Location
Time Period
Advisors
Original Date
Digitization Date
Issue Date
2008-03-24
Type
Article
Genre
Keywords
Forensic science,DNA typing,Polymerase chain reaction,Restriction fragment length polymorphism,Membrane,Amplification
Subjects (LCSH)
Citation
Steadman, Shelly A.; McDonald, J. David; Andrews, John S.; Watson, Nigel D. 2008. Recovery and STR amplification of DNA from RFLP membranes. Journal of Forensic Sciences, v.53 no.2 pp.349-358
Abstract
Restriction fragment length polymorphism (RFLP) techniques were utilized in the forensic DNA community until the mid 1990s when less labor-intensive polymerase chain reaction short tandem repeat (PCR STR) techniques became available. During the transition from RFLP technology to PCR-based STR platforms, a method for comparing RFLP profiles to STR profiles was not developed. While the preferred approach for applying new technology to old cases would be to analyze the original biological stain, this is not always possible. For unsolved cases that previously underwent RFLP analysis, the only DNA remaining may be restriction cut and bound to nylon membranes. These studies investigate several methods for obtaining STR profiles from membrane bound DNA, including removal of bound DNA with bases, acids, detergents, various chemicals, and conventional cell extraction solutions. Direct multiplex STR amplification of template in the membrane-bound state was also explored. A partial STR profile was obtained from DNA that was recovered from an archived membrane using conventional extraction buffer components, indicating promise for recovering useful STR information from RFLP membranes that have been maintained in long-term frozen storage.
Table of Contents
Description
Click on the DOI link to access the article (may not be free).
Publisher
American Academy of Forensic Sciences
Journal
Book Title
Series
Journal of Forensic Sciences;v.53 no.2