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Exploring the role of ABA in plant defense against the necrotrophic pathogen Macrophomina phaseolina
Hefel, Adam
Hefel, Adam
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2015-04-24
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Hefel, Adam. Exploring the Role of ABA in Plant Defense Against the Necrotrophic Pathogen Macrophomina phaseolina. --In Proceedings: 11th Annual Symposium on Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p. 50
Abstract
Macrophomina phaseolina is a major agricultural pest causing reduced crop yield across many
continents including Asia, Europe, North, and South America. As a necrotrophic fungus, M.
phaseolina destroys plant tissue to acquire nutrients, causing the disease charcoal rot in many
plant hosts including agriculturally important legumes such as soybean and alfalfa. Charcoal Rot
leads to leaf yellowing, root rot, and eventually plant death in infected plants. The disease can
result in significantly reduced crop yield especially during hot and dry years when plants suffer
from drought stress.
Plants can respond to drought stress with guard cell closure, effectively limiting water loss. This
process is driven by the phytohormone abscisic acid (ABA) which is produced in drought
stressed plants to induce drought tolerance. ABA-treated plants are more tolerant to drought
stress, whereas, plants defective in ABA synthesis or ABA signaling are susceptible to drought.
However, the effects of drought resistance on infection are unclear, as some drought resistant
cultivars of soybean have demonstrated partial resistance to M. phaseolina, and others show
increased susceptibility. To investigate if promoting a drought response using exogenous ABA
application may aid in plant defense against M. phaseolina, plants of the model legume
Medicago truncatula were grown in a sterile environment on media containing varying
concentrations of ABA. Using M. phaseolina infected wheat seeds; plants were inoculated with
the pathogen and then observed for the development of chlorotic and necrotic tissue as a measure
of disease severity. Anova analysis and Tukey's honesty significance test of this data suggest
that treating plants with low doses of ABA (10-20 ?M) significantly accelerated disease
progression, suggesting that ABA production and signaling may be detrimental to plants infected
with M. phaseolina.
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Presented to the 11th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Heskett Center, Wichita State University, April 24, 2015.
Research completed at Department of Biology, Fairmount College of Liberal Arts and Sciences
Research completed at Department of Biology, Fairmount College of Liberal Arts and Sciences
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Wichita State University. Graduate School
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GRASP
v.11
v.11