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Testing the role of exercise training in aging-related stem cell dysregulation
Rajagopal, Andriana Madhavan, Rahul Clark, Elsie Padamati, Divya Loganathan, Rajprasad
Rajagopal, Andriana
Madhavan, Rahul
Clark, Elsie
Padamati, Divya
Loganathan, Rajprasad
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2024-04-12
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Rajagopal, Andriana; Madhavan, Rahul; Clark, Elsie; Padamati, Divya; Loganathan, Rajprasad. 2024. Testing the role of exercise training in aging-related stem cell dysregulation. -- In Proceedings: 23rd Annual Undergraduate Research and Creative Activity Forum. Wichita, KS: Wichita State University, p. 23.
Abstract
The effects of exercise and its roles in the regulation of stem cell maintenance and function are unknown and could have profound implications for our understanding of stem cell health. This project aims to investigate the response of the JAK-STAT signaling pathway, a pro-stem cell homeostasis pathway in the testis niche, to exercise training. We have built an exercise apparatus for fruit flies, Power Tower version 2.0, to allow the flies to undergo three weeks of exercise training. Their exercise intensity will gradually increase from weeks 1 through 3. The training protocol takes advantage of the Drosophila's innate negative geotaxis ability (the directional movement against gravity) by forcing them to climb continuously in the container or until the apparatus is turned off. We expect to test the hypothesis that exercise training will correlate with an upregulation of JAK-STAT signaling and an increase in testis stem cell viability within the treatment group. In addition to building the apparatus, we have also optimized the protocol for antibody staining and evaluation of cell types in the Drosophila testis stem cell niche. We will provide an overview of the experimental plan designed to test our hypothesis that exercise attenuates aging-related stem cell dysfunction and present the preliminary data obtained from this ongoing investigation.
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Presented to the 23rd Undergraduate Research and Creative Activity Forum (URCAF) held at the Rhatigan Student Center, Wichita State University, April 12, 2024.
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Wichita State University
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URCAF;v.23