Purification and partial characterization of beef liver gluconolactonase
Bailey, G.D. Roberts, B.D. Buess, Charles M. Carper, W. Robert
Bailey, G.D.
Roberts, B.D.
Buess, Charles M.
Carper, W. Robert
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1979
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Article
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Biochemistry,Bovines,Liver glucose
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Bailey GD, Roberts BD, Buess CM, Carper WR. Purification and partial characterization of beef liver gluconolactonase. Arch Biochem Biophys. 1979 Feb;192(2):482-8. doi: 10.1016/0003-9861(79)90118-8. PMID: 35106.
Abstract
Gluconolactonase is isolated and purified from beef liver. The molecular weight is estimated at 233,000 and that of its six similar subunits is 39,400. The pH maximum is 7.1 in 50 mm Tris-acetate buffer at 27 °C. Km and Vm values of 9.1 mm and 1.62 mmol/min/ mg, respectively, were obtained at 27 °C in 50 mm Tris-HCl buffer. This enzyme requires a divalent metal for activity, with manganese being preferred over magnesium. A subcellular fractionation study indicates that gluconolactonase is located primarily in the cytosol, and its hepatic concentration is 2.3 ?mol/kg of hepatic tissue. © 1979.
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Archives of Biochemistry and Biophysics
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0003-9861