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Nanoporous impedemetric biosensor for detection of trace atrazine from water samples
Pichetsurnthorn, Pie Vattipalli, Krishna Prasad, Shalini
Pichetsurnthorn, Pie
Vattipalli, Krishna
Prasad, Shalini
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2012-02-15
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Article
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Keywords
Trace atrazine detection,River water,Drinking water,Electrochemical impedance spectroscopy,Nanoporous alumina,Label-free detection
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Citation
Pichetsurnthorn, P., K. Vattipalli, and S. Prasad. 2012. "Nanoporous impedemetric biosensor for detection of trace atrazine from water samples". Biosensors and Bioelectronics. 32 (1): 155-162.
Abstract
Trace contamination of ground water sources has been a problem ever since the introduction of high-soil-mobility pesticides, one such example is atrazine. In this paper we present a novel nanoporous portable bio-sensing device that can identify trace contamination of atrazine through a label-free assay. We have designed a pesticide sensor comprising of a nanoporous alumina membrane integrated with printed circuit board platform. Nanoporous alumina in the biosensor device generates a high density array of nanoscale confined spaces. By leveraging the size based immobilization of atrazine small molecules we have designed electrochemical impedance spectroscopy based biosensor to detect trace amounts of atrazine. We have calibrated the sensor using phosphate buffered saline and demonstrated trace detection from river and bottled drinking water samples. The limit of detection in all the three cases was in the femtogram/mL (fg/mL) (parts-per-trillion) regime with a dynamic range of detection spanning from 10 fg/mL to 1 ng/mL (0.01 ppt to 1 ppm). The selectivity of the device was tested using a competing pesticide; malathion and selectivity in detection was observed in the fg/mL regime in all the three cases.
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Elsevier
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Biosensors and Bioelectronics;2012:, v.32, no.1
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0956-5663
1873-4235
1873-4235