Investigation of intramolecular proton migration in a series of model, metal-cationized tripeptides using in situ generation of an isotope label
Bulleigh, Kellis Howard, Angela Do, Trang Wu, Qun Anbalagan, Victor Van Stipdonk, Michael J.
Bulleigh, Kellis
Howard, Angela
Do, Trang
Wu, Qun
Anbalagan, Victor
Van Stipdonk, Michael J.
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2006-01-01
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Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.
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Rapid communications in mass spectrometry : RCM. 2006; 20(2): 227-32.
Abstract
In this study we used an isotope label, generated in situ, to investigate intramolecular proton migration or scrambling during formation of [b(2)+17+Li](+) products by collision-induced dissociation (CID) of Li(+)-cationized tripeptides. To generate the isotope label, we used a McLafferty-type rearrangement of N-terminally acetylated, C-terminal peptide tert-butyl esters in which all amide positions were exchanged with deuterium. Using a set of small, model peptides, we show that intramolecular proton scrambling occurs during CID, particularly amongst adjacent sites along a peptide backbone, on the time scales employed for low-energy collisional activation in an ion-trap mass spectrometer.
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John Wiley and Sons
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Rapid communications in mass spectrometry : RCM
Rapid Commun. Mass Spectrom.
Rapid Commun. Mass Spectrom.
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0951-4198