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Development of a method to investigate intra-molecular proton migration during collision induced dissociation of gas-phase peptides

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dc.contributor.advisor Van Stipdonk, Michael J. en_US
dc.contributor.author Wu, Qun
dc.date.accessioned 2007-08-23T13:54:49Z
dc.date.available 2007-08-23T13:54:49Z
dc.date.issued 2005-12
dc.identifier.isbn 9780542758041
dc.identifier.other t05041
dc.identifier.other AAT 1436592:UMI
dc.identifier.uri http://hdl.handle.net/10057/779
dc.description Thesis (M.S.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Chemistry. en
dc.description "December 2005." en
dc.description.abstract Use of a combination of hydrogen/deuterium (H/D) exchange, mass spectrometry (MS) and/or CID is a rapidly evolving approach to the investigation of protein structure and conformation and the dynamics of conformational change. It has been noted, however, interpretation of CID MS data on partially deuterated peptide ions can, in principle, be hampered by both inter- and intra-molecular migration of hydrogen and deuterium atoms. In this study, an isotope tracer was produced in situ by a McLafferty-type rearrangement of C-terminal peptide tert -butyl esters. This rearrangement generates a peptide with a C-terminal acid group with an isotope label. The label can then be traced through multiple CID stages to determine the amount of intra-molecular migration and scrambling of protons among exchangeable sites. Experimental results from a series of Li + cationized sarcosine substitution peptides show that the C-terminal acid and amide positions, and the amide position adjacent to the C-terminus, allow the exchange of the isotope label. Transfer to the amide position at the N-terminus, the position for which the distance from the site at which the isotope tracer was initially generated is the greatest, appears to be less favored. The influence of amino acids and cations on intramolecular proton migration was also investigated. Three series of peptides AcSarGXOtBu, AcGSarXOtBu, AcSarSarXOtBu (where X was glycine(G), alanine(A), valine(V), leucine(L), phenylalanine(F)) together with three cations Li + , Na + and Ag + were used in this study. The results show that amino acids and cations have only a slight effect on the amount of proton migration observed. en
dc.format.extent 456071 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.rights Copyright Qun Wu, 2005. All rights reserved. en
dc.subject.lcsh Electronic dissertations en
dc.title Development of a method to investigate intra-molecular proton migration during collision induced dissociation of gas-phase peptides en
dc.type Thesis en

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