Analysis of differential glycosylation patterns of human FSH
Chambers, Carrie Anne
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Chambers, Carrie (2010). Analysis of differential glycosylation patterns of human FSH. -- In Proceedings: 6th Annual Symposium: Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University, p. 21-22
Follicle stimulating hormone (FSH) is a glycoprotein hormone with subunits α and β, and is required for gamete development. Differential glycosylation may produce diglycosylated FSH with higher biological activity, and is suspected to be generated via the action of oligosaccharyltransferase(OST) isoforms. Signal peptide hydrophobicity of α and β maycontribute to selective usage of OST, and hence modulate N-glycosylation. We hypothesize that N-glycosylation of FSH subunits is regulated via differential interactions between OST isoforms and subunit signal peptides and modulated by estrogen. To test our hypothesis, we will engineer chimeric hFSH subunits by swapping the signal peptide sequences of α and β. Constructs with the chimeric sequences will be transfected into cell lines, and expressed FSH will be examined.
First Place winner of oral presentations at the 6th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Hughes Metropolitan Complex, Wichita State University, April 23, 2010.
Research completed at the Department of Biological Sciences, College of Liberal Arts and Sciences