The long- term goal of the project is to develop an environmentally safe, sensitive method for the detection of reactive oxygen species. The current hypothesis is that the biosynthetic incorporation of [2,5-H2]-phenylalanine into the UV sensitive fluorescent variant Tyr66Phe of the green fluorescent protein will allow the sensing of reactive oxygen species by fluorescence

Paper presented to the 3rd Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Hughes Metropolitan Complex, Wichita State University, April 27, 2007.Research completed at the Department of Chemistry, College of Liberal Arts and Sciences