Affinity chromatography of glucose dehydrogenase

SOAR Repository

Show simple item record

dc.contributor Wichita State University. Department of Chemistry en_US
dc.contributor.author Carper, W. Robert en_US
dc.contributor.author Groutas, William C. en_US
dc.contributor.author Coffin, D.B. en_US
dc.date.accessioned 2012-02-06T17:17:04Z
dc.date.available 2012-02-06T17:17:04Z
dc.date.issued 1988-01-15 en_US
dc.identifier 3350116 en_US
dc.identifier 0376547 en_US
dc.identifier DK 38853-01 en_US
dc.identifier.citation Experientia. 1988 Jan 15; 44(1): 29-32. en_US
dc.identifier.issn 0014-4754 en_US
dc.identifier.uri http://hdl.handle.net/10057/4390
dc.description Full text of this article is not available in SOAR. en_US
dc.description.abstract Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies. en_US
dc.description.sponsorship NIDDK NIH HHS en_US
dc.format.extent 29-32 en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Experientia en_US
dc.relation.ispartofseries Experientia en_US
dc.source NLM en_US
dc.subject Research Support, Non-U.S. Gov't en_US
dc.subject Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Carbohydrate Dehydrogenases/isolation & purification en_US
dc.subject.mesh Chromatography en_US
dc.subject.mesh Electrophoresis, Polyacrylamide Gel en_US
dc.subject.mesh Endoplasmic Reticulum/enzymology en_US
dc.subject.mesh Glucose 1-Dehydrogenase en_US
dc.subject.mesh Glucose Dehydrogenases/isolation & purification en_US
dc.subject.mesh Isoelectric Focusing en_US
dc.subject.mesh Kinetics en_US
dc.subject.mesh Liver/enzymology en_US
dc.subject.mesh NAD/metabolism en_US
dc.subject.mesh NADP/metabolism en_US
dc.subject.mesh Swine en_US
dc.subject.mesh Glucose Dehydrogenases/metabolism en_US
dc.title Affinity chromatography of glucose dehydrogenase en_US
dc.type Article en_US
dc.coverage.spacial Switzerland en_US
dc.description.version peer reviewed en_US
dc.rights.holder Copyright © 1988, Birkhäuser Basel en_US

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search SOAR

Advanced Search


My Account