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Immobilization and electrochemical redox behavior of cytochrome c on fullerene film-modified electrodes

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dc.contributor Wichita State University. Department of Chemistry en_US
dc.contributor.author D'Souza, Francis en_US
dc.contributor.author Rogers, Lisa M. en_US
dc.contributor.author O'Dell, Erin S. en_US
dc.contributor.author Kochman, Agnieszka en_US
dc.contributor.author Kutner, Wlodzimierz en_US
dc.date.accessioned 2012-02-06T17:15:10Z
dc.date.available 2012-02-06T17:15:10Z
dc.date.issued 2005-04-01 en_US
dc.identifier 15833700 en_US
dc.identifier 100953583 en_US
dc.identifier S1567-5394(04)00208-7 en_US
dc.identifier.citation Bioelectrochemistry (Amsterdam, Netherlands). 2005 Apr; 66(1-2): 35-40. en_US
dc.identifier.issn 1567-5394 en_US
dc.identifier.uri http://dx.doi.org/10.1016/j.bioelechem.2004.03.013 en_US
dc.identifier.uri http://hdl.handle.net/10057/4217
dc.description Click on the DOI link below to access the article (may not be free). en_US
dc.description.abstract Two different fullerene film-modified electrodes were prepared and used for surface immobilization and electrochemical property investigation of horse heart cytochrome c (cyt c). Both a pristine fullerene film and fullerene-palladium (C(60)-Pd) polymer film-modified platinum, glassy carbon and indium-tin-oxide (ITO) electrodes were used. The immobilized cyt c was characterized by piezoelectric microgravimetry at a quartz crystal microbalance (QCM), UV-visible absorption, and X-ray photoelectron spectroscopy (XPS), as well as cyclic voltammetry (CV) techniques. The UV-visible spectral studies revealed a small blue shift of both the Soret and Q band of the heme moiety of cyt c, immobilized on the C(60)-Pd polymer film-modified ITO electrode, as compared to the bands of cyt c in solution suggesting that molecules of cyt c are densely packed onto the surface of the modified electrode. The CV studies revealed a quasi-reversible electrode behavior of the heme moiety indicating the occurrence of kinetically hindered electron transfer. A good agreement was found between the values of cyt c electrode surface coverage determined by piezoelectric microgravimetry and cyclic voltammetry. For piezoelectric microgravimetry, these values ranged from 0.5 x 10(-10) to 2.5 x 10(-10) mol cm(-2), depending upon the amount of cyt c present in solution and the time allowed for immobilization, which compared with a value of 3.6+/-0.4 x 10(-10) mol cm(-2) determined by CV. The possible mechanisms of cyt c immobilization on the C(60) film and C(60)-Pd film-modified electrodes are also discussed. en_US
dc.format.extent 35-40 en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Bioelectrochemistry (Amsterdam, Netherlands) en_US
dc.relation.ispartofseries Bioelectrochemistry en_US
dc.source NLM en_US
dc.subject Research Support, Non-U.S. Gov't en_US
dc.subject Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Adsorption en_US
dc.subject.mesh Cytochromes c/chemistry en_US
dc.subject.mesh Electrodes en_US
dc.subject.mesh Enzymes, Immobilized/chemistry en_US
dc.subject.mesh Fullerenes/chemistry en_US
dc.subject.mesh Oxidation-Reduction en_US
dc.subject.mesh Palladium/chemistry en_US
dc.subject.mesh Spectrophotometry, Ultraviolet en_US
dc.title Immobilization and electrochemical redox behavior of cytochrome c on fullerene film-modified electrodes en_US
dc.type Article en_US
dc.coverage.spacial Netherlands en_US
dc.description.version peer reviewed en_US
dc.rights.holder Copyright © 2005, Elsevier en_US

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