| dc.contributor |
Wichita State University. Department of Chemistry |
en_US |
| dc.contributor.author |
D'Souza, Francis |
en_US |
| dc.contributor.author |
Rogers, Lisa M. |
en_US |
| dc.contributor.author |
O'Dell, Erin S. |
en_US |
| dc.contributor.author |
Kochman, Agnieszka |
en_US |
| dc.contributor.author |
Kutner, Wlodzimierz |
en_US |
| dc.date.accessioned |
2012-02-06T17:15:10Z |
|
| dc.date.available |
2012-02-06T17:15:10Z |
|
| dc.date.issued |
2005-04-01 |
en_US |
| dc.identifier |
15833700 |
en_US |
| dc.identifier |
100953583 |
en_US |
| dc.identifier |
S1567-5394(04)00208-7 |
en_US |
| dc.identifier.citation |
Bioelectrochemistry (Amsterdam, Netherlands). 2005 Apr; 66(1-2): 35-40. |
en_US |
| dc.identifier.issn |
1567-5394 |
en_US |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.bioelechem.2004.03.013 |
en_US |
| dc.identifier.uri |
http://hdl.handle.net/10057/4217 |
|
| dc.description |
Click on the DOI link below to access the article (may not be free). |
en_US |
| dc.description.abstract |
Two different fullerene film-modified electrodes were prepared and used for surface immobilization and electrochemical property investigation of horse heart cytochrome c (cyt c). Both a pristine fullerene film and fullerene-palladium (C(60)-Pd) polymer film-modified platinum, glassy carbon and indium-tin-oxide (ITO) electrodes were used. The immobilized cyt c was characterized by piezoelectric microgravimetry at a quartz crystal microbalance (QCM), UV-visible absorption, and X-ray photoelectron spectroscopy (XPS), as well as cyclic voltammetry (CV) techniques. The UV-visible spectral studies revealed a small blue shift of both the Soret and Q band of the heme moiety of cyt c, immobilized on the C(60)-Pd polymer film-modified ITO electrode, as compared to the bands of cyt c in solution suggesting that molecules of cyt c are densely packed onto the surface of the modified electrode. The CV studies revealed a quasi-reversible electrode behavior of the heme moiety indicating the occurrence of kinetically hindered electron transfer. A good agreement was found between the values of cyt c electrode surface coverage determined by piezoelectric microgravimetry and cyclic voltammetry. For piezoelectric microgravimetry, these values ranged from 0.5 x 10(-10) to 2.5 x 10(-10) mol cm(-2), depending upon the amount of cyt c present in solution and the time allowed for immobilization, which compared with a value of 3.6+/-0.4 x 10(-10) mol cm(-2) determined by CV. The possible mechanisms of cyt c immobilization on the C(60) film and C(60)-Pd film-modified electrodes are also discussed. |
en_US |
| dc.format.extent |
35-40 |
en_US |
| dc.language.iso |
eng |
en_US |
| dc.publisher |
Elsevier |
en_US |
| dc.relation.ispartofseries |
Bioelectrochemistry (Amsterdam, Netherlands) |
en_US |
| dc.relation.ispartofseries |
Bioelectrochemistry |
en_US |
| dc.source |
NLM |
en_US |
| dc.subject |
Research Support, Non-U.S. Gov't |
en_US |
| dc.subject |
Research Support, U.S. Gov't, Non-P.H.S. |
en_US |
| dc.subject |
Research Support, U.S. Gov't, P.H.S. |
en_US |
| dc.subject.mesh |
Adsorption |
en_US |
| dc.subject.mesh |
Cytochromes c/chemistry |
en_US |
| dc.subject.mesh |
Electrodes |
en_US |
| dc.subject.mesh |
Enzymes, Immobilized/chemistry |
en_US |
| dc.subject.mesh |
Fullerenes/chemistry |
en_US |
| dc.subject.mesh |
Oxidation-Reduction |
en_US |
| dc.subject.mesh |
Palladium/chemistry |
en_US |
| dc.subject.mesh |
Spectrophotometry, Ultraviolet |
en_US |
| dc.title |
Immobilization and electrochemical redox behavior of cytochrome c on fullerene film-modified electrodes |
en_US |
| dc.type |
Article |
en_US |
| dc.coverage.spacial |
Netherlands |
en_US |
| dc.description.version |
peer reviewed |
en_US |
| dc.rights.holder |
Copyright © 2005, Elsevier |
en_US |