Archaeal diversity at the great salt plains of Oklahoma described by cultivation and molecular analyses

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dc.contributor.author Caton, Todd M. en_US
dc.contributor.author Santos-Caton, Ingrid R. en_US
dc.contributor.author Witte, Lisa R. en_US
dc.contributor.author Schneegurt, Mark A. en_US
dc.date.accessioned 2012-01-24T17:49:57Z
dc.date.available 2012-01-24T17:49:57Z
dc.date.issued 2009-10 en_US
dc.identifier 19306116 en_US
dc.identifier P20 RR16475 en_US
dc.identifier 7500663 en_US
dc.identifier.citation Microbial ecology. 2009 Oct; 58(3): 519-28. en_US
dc.identifier.issn 1432-184X en_US
dc.identifier.issn 0095-3628 en_US
dc.identifier.uri http://dx.doi.org/10.1007/s00248-009-9507-y
dc.identifier.uri http://hdl.handle.net/10057/4205
dc.description Click on the DOI link below to access the article (may not be free). en_US
dc.description.abstract The Great Salt Plains of Oklahoma is a natural inland terrestrial hypersaline environment that forms evaporite crusts of mainly NaCl. Previous work described the bacterial community through the characterization of 105 isolates from 46 phylotypes. The current report describes the archaeal community through both microbial isolation and culture-independent techniques. Nineteen distinct archaea were isolated, and ten were characterized phenetically. Included were isolates phylogenetically related to Haloarcula, Haloferax, Halorubrum, Haloterrigena, and Natrinema. The isolates were aerobic, non-motile, Gram-negative organisms and exhibited little capacity for fermentation. All of the isolates were halophilic, with most requiring at least 15% salinity for growth, and all grew at 30% salinity. The isolates were mainly mesothermic and could grow at alkaline pH (8.5). A 16S rRNA gene library was generated by polymerase chain reaction amplification of direct soil DNA extracts, and 200 clones were sequenced and analyzed. At 99% and 94% sequence identity, 36 and 19 operational taxonomic units (OTUs) were detected, respectively, while 53 and 22 OTUs were estimated by Chao1, respectively. Coverage was relatively high (100% and 59% at 89% and 99% sequence identity, respectively), and the Shannon Index was 3.01 at 99% sequence identity, comparable to or somewhat lower than hypersaline habitats previously studied. Only sequences from Euryarchaeota in the Halobacteriales were detected, and the strength of matches to known sequences was generally low, most near 90% sequence identity. Large clusters were observed that are related to Haloarcula and Halorubrum. More than two-thirds of the sequences were in clusters that did not have close relatives reported in public databases. en_US
dc.description.sponsorship NCRR NIH HHS en_US
dc.language.iso eng en_US
dc.publisher Springer New York en_US
dc.relation.ispartofseries Microbial ecology en_US
dc.source NLM en_US
dc.subject Research Support, N.I.H., Extramural en_US
dc.subject Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject.mesh Biodiversity en_US
dc.subject.mesh DNA, Archaeal/genetics en_US
dc.subject.mesh Gene Library en_US
dc.subject.mesh Halobacteriaceae/classification en_US
dc.subject.mesh Oklahoma en_US
dc.subject.mesh Phylogeny en_US
dc.subject.mesh RNA, Ribosomal, 16S/genetics en_US
dc.subject.mesh Salinity en_US
dc.subject.mesh Sequence Analysis, DNA en_US
dc.subject.mesh Sodium Chloride en_US
dc.subject.mesh Soil Microbiology en_US
dc.subject.mesh Halobacteriaceae/genetics en_US
dc.subject.mesh Halobacteriaceae/isolation & purification en_US
dc.title Archaeal diversity at the great salt plains of Oklahoma described by cultivation and molecular analyses en_US
dc.type Article en_US
dc.description.version peer reviewed en_US
dc.rights.holder Copyright © 2009, Springer Science+Business Media, LLC en_US

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