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Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR

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dc.contributor.author Chambers, Carrie Anne en_US
dc.contributor.author Shuai, Bin en_US
dc.date.accessioned 2012-01-24T17:49:35Z
dc.date.available 2012-01-24T17:49:35Z
dc.date.issued 2009 en_US
dc.identifier 19591667 en_US
dc.identifier P20 RR016475 en_US
dc.identifier 100967807 en_US
dc.identifier.citation BMC plant biology. 2009; 9: 87. en_US
dc.identifier.issn 1471-2229 en_US
dc.identifier.uri http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715406/pdf/1471-2229-9-87.pdf
dc.identifier.uri http://hdl.handle.net/10057/4185
dc.description Click on the link below to access the article (may not be free). en_US
dc.description.abstract MicroRNAs (miRNAs) are approximately 22-nt small non-coding RNAs that regulate the expression of specific target genes in many eukaryotes. In higher plants, miRNAs are involved in developmental processes and stress responses. Sexual reproduction in flowering plants relies on pollen, the male gametophyte, to deliver sperm cells to fertilize the egg cell hidden in the embryo sac. Studies indicated that post-transcriptional processes are important for regulating gene expression during pollen function. However, we still have very limited knowledge on the involved gene regulatory mechanisms. Especially, the function of miRNAs in pollen remains unknown. en_US
dc.description.sponsorship NCRR NIH HHS en_US
dc.language.iso eng en_US
dc.publisher BioMed Central Ltd. en_US
dc.relation.ispartofseries BMC plant biology en_US
dc.source NLM en_US
dc.subject Research Support, N.I.H., Extramural en_US
dc.subject Research Support, Non-U.S. Gov't en_US
dc.subject.mesh Arabidopsis/genetics en_US
dc.subject.mesh Gene Expression Profiling en_US
dc.subject.mesh Gene Expression Regulation, Plant en_US
dc.subject.mesh MicroRNAs/metabolism en_US
dc.subject.mesh Oligonucleotide Array Sequence Analysis en_US
dc.subject.mesh Pollen/genetics en_US
dc.subject.mesh RNA Interference en_US
dc.subject.mesh RNA, Plant/metabolism en_US
dc.subject.mesh Reverse Transcriptase Polymerase Chain Reaction en_US
dc.subject.mesh Sequence Analysis, RNA en_US
dc.subject.mesh Arabidopsis/metabolism en_US
dc.subject.mesh Pollen/metabolism en_US
dc.title Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR en_US
dc.type Article en_US
dc.description.version peer reviewed en_US
dc.rights.holder Copyright © 2009 Chambers and Shuai en_US

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