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dc.contributor.authorSadiev, Sagypashen_US
dc.contributor.authorTaylor, Alanen_US
dc.date.accessioned2012-01-24T17:49:25Z
dc.date.available2012-01-24T17:49:25Z
dc.date.issued1996-08en_US
dc.identifier8862806en_US
dc.identifier8306785en_US
dc.identifier.citationBioTechniques. 1996 Aug; 21(2): 233-5.en_US
dc.identifier.issn0736-6205en_US
dc.identifier.urihttp://www.biotechniques.com/multimedia/archive/00011/96212bm14_11503a.pdf
dc.identifier.urihttp://hdl.handle.net/10057/4165
dc.descriptionClick on the link below to access the article (may not be free).en_US
dc.description.abstractThe increased use of plasmid DNAs in recombinant DNA technology, coupled with the growing popularity of double-stranded DNA sequencing, necessitates the development of rapid, inexpensive and efficient procedures for the isolation of highly purified plasmids. Numerous miniprep procedures have been described for preparing plasmid DNAs (1,2,4,5,8,9). A quick and efficient alternative method for the preparation of large quantities of purified plasmid DNA is presented in this report. Plasmid isolated by this method is of sufficient quality for use in DNA sequencing reactions and other molecular biological techniques. This procedure provides an attractive alternative to more expensive and/or timeconsuming methods currently used to prepare plasmid DNA.en_US
dc.language.isoengen_US
dc.publisherInforma Healthcareen_US
dc.relation.ispartofseriesBioTechniquesen_US
dc.sourceNLMen_US
dc.subjectResearch Support, Non-U.S. Gov'ten_US
dc.subjectResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshDNA/geneticsen_US
dc.subject.meshDNA, Bacterial/geneticsen_US
dc.subject.meshDNA, Recombinant/geneticsen_US
dc.subject.meshEscherichia coli/geneticsen_US
dc.subject.meshGenetic Vectors/geneticsen_US
dc.subject.meshNucleic Acid Denaturationen_US
dc.subject.meshSequence Analysis, DNA/methodsen_US
dc.subject.meshTemplates, Geneticen_US
dc.subject.meshDNA/isolation & purificationen_US
dc.subject.meshDNA, Bacterial/isolation & purificationen_US
dc.subject.meshDNA, Recombinant/isolation & purificationen_US
dc.titleAlternative method for isolation of double-stranded template for DNA sequencingen_US
dc.typeArticleen_US
dc.description.versionpeer revieweden_US


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