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Alternative method for isolation of double-stranded template for DNA sequencing

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dc.contributor.author Sadiev, Sagypash en_US
dc.contributor.author Taylor, Alan en_US
dc.date.accessioned 2012-01-24T17:49:25Z
dc.date.available 2012-01-24T17:49:25Z
dc.date.issued 1996-08 en_US
dc.identifier 8862806 en_US
dc.identifier 8306785 en_US
dc.identifier.citation BioTechniques. 1996 Aug; 21(2): 233-5. en_US
dc.identifier.issn 0736-6205 en_US
dc.identifier.uri http://www.biotechniques.com/multimedia/archive/00011/96212bm14_11503a.pdf
dc.identifier.uri http://hdl.handle.net/10057/4165
dc.description Click on the link below to access the article (may not be free). en_US
dc.description.abstract The increased use of plasmid DNAs in recombinant DNA technology, coupled with the growing popularity of double-stranded DNA sequencing, necessitates the development of rapid, inexpensive and efficient procedures for the isolation of highly purified plasmids. Numerous miniprep procedures have been described for preparing plasmid DNAs (1,2,4,5,8,9). A quick and efficient alternative method for the preparation of large quantities of purified plasmid DNA is presented in this report. Plasmid isolated by this method is of sufficient quality for use in DNA sequencing reactions and other molecular biological techniques. This procedure provides an attractive alternative to more expensive and/or timeconsuming methods currently used to prepare plasmid DNA. en_US
dc.language.iso eng en_US
dc.publisher Informa Healthcare en_US
dc.relation.ispartofseries BioTechniques en_US
dc.source NLM en_US
dc.subject Research Support, Non-U.S. Gov't en_US
dc.subject Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject.mesh DNA/genetics en_US
dc.subject.mesh DNA, Bacterial/genetics en_US
dc.subject.mesh DNA, Recombinant/genetics en_US
dc.subject.mesh Escherichia coli/genetics en_US
dc.subject.mesh Genetic Vectors/genetics en_US
dc.subject.mesh Nucleic Acid Denaturation en_US
dc.subject.mesh Sequence Analysis, DNA/methods en_US
dc.subject.mesh Templates, Genetic en_US
dc.subject.mesh DNA/isolation & purification en_US
dc.subject.mesh DNA, Bacterial/isolation & purification en_US
dc.subject.mesh DNA, Recombinant/isolation & purification en_US
dc.title Alternative method for isolation of double-stranded template for DNA sequencing en_US
dc.type Article en_US
dc.description.version peer reviewed en_US

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